摘要
目的：建立测定苦石莲胶囊中Neocaesalpin L含量的反相高效液相色谱法。方法：色谱柱为伊力特ODS C18（250 mm×4.6 mm,5μm）;流动相为乙腈-0.5%磷酸水梯度洗脱;检测波长为210 nm;柱温为30℃;流速为0.8 m L·min-1。结果：Neocaesalpin L在0.74~3.16μg线性关系良好,r=0.999 9,Neocaesalpin L平均回收率为96.76%,RSD=1.59%（n=9）。结论：该方法简单,专属性强,重复性好,可有效控制苦石莲胶囊的质量。
Objective： To establish an RP-HPLC method for quantitative determination of neocaesalpin L in Caesalpinia minax capsule. Methods： The separation was performed on a Elite ODS C18Column（ 250 mm × 4. 6 mm,5 μm）acetonitril-0. 5% H3PO4 was used as the mobile phase. The detection wavelength was at 210 nm. The temperature was maintained at 30 ℃. The flow rate was 0. 8 m L·min-1. Results： The concentration of neocaesalpin L was linear within the range of 0. 74-3. 16 μg（ r = 0. 999 9）. The average recovery and RSD of the method were 96. 76% and 1. 59%（ n = 9）.Conclusion： The method is accurate,specific,reproducible which can effectively be used in quality control of neocaesalpin L in Caesalpinia minax capsule.
出处
《中国现代中药》
CAS
2015年第9期962-964,共3页
Modern Chinese Medicine
基金
广西壮族自治区中医药管理局中医药科技专项(GXYZ1112)
桂科转(1298009-22)
南宁市科技攻关与新产品试制(201101088C)
