摘要
目的了解上海市闵行区初级农产品中金黄色葡萄球菌的污染状况及毒力基因的携带情况。方法按国标方法,采用科玛嘉显色培养基,对初级农产品中的金黄色葡萄球菌进行分离、生化鉴定,收集的菌株采用实时荧光PCR方法检测血浆凝固酶基因coa,耐热核酸酶基因nuc,粘附素基因clfA和肠毒素基因sea、seb、sec、sed、see。结果 206份食品中检出金黄色葡萄球菌43株,检出率为20.87%;43株金黄色葡萄球菌均含有coa、nuc和clfA基因,而see基因全部缺失,其余4个毒力基因则表现为部分缺失。结论本地区初级农产品中存在金黄色葡萄球菌的污染,生肉禽类食品中金黄色葡萄球菌污染比较严重,应加强监督管理;金黄色葡萄球菌菌株均包含有coa毒力基因,可以作为一项检测其致病力的指标。
Objective To investigate the contamination and the virulence genes of Staphylococcus aureus in the primary farm products of Minhang District, Shanghai. Methods CHROMagar color medium and API S. aureus were used for separation and biochemical identification of S. aureus according to national standard. Virulence genes of coa, nuc, clfA, sea, seb, sec, sed and see were detected by real-time PCR methods. Results 43 isolates were identified from 206 S. aureus samples, and the detection rate was 20.87%. All the identified strains had coa, nuc and clfA virulence genes, but no see virulence gene was found, and the remaining four virulence genes expressions were partially missing. Conclusion The contamination of S. aureus in the primary farm products existed in Minhang district of Shanghai. Supervision and management should be strengthened for raw meat and poultry for serious contamination. All S. aureus strains contained coa virulence gene. It could be used as a target for detecting virulence genes.
出处
《中国食品卫生杂志》
北大核心
2014年第5期434-437,共4页
Chinese Journal of Food Hygiene
基金
上海市闵行区科委自然基金项目(2011MHZ55)
关键词
金黄色葡萄球菌
污染
毒力基因
食源性致病菌
初级农产品
农产品
食品安全
Staphylococcus aureus
contamination
virulence genes
foodborne pathogenic bacteria
primary farmproducts
farm products
food safety
