摘要
目的:描述生物被膜态金黄色葡萄球菌的代谢轮廓特征,以发展新的金黄色葡萄球菌生物被膜的鉴定和处理方法。方法:使用1H核磁共振波谱法对生物被膜培养物进行非靶标分析;使用Chenomx软件鉴定化合物;使用主成分分析法分析主要代谢物变化。结果:在生物被膜培养物中鉴定出41种化合物。在生物被膜生长期间,金黄色葡萄球菌大致摄取了10种化合物,包括葡萄糖、精氨酸、天冬氨酸、苏氨酸、乙酰乙酸、甲酸、琥珀酸、甘油、甜菜碱和肉碱;积累了14种代谢物,包括1,3-二羟丙酮、2-氨基丁酸、鸟氨酸、3-羟基异戊酸、3-甲基戊二酸、乙酸、异丁酸、异戊酸、乳酸、焦谷氨酸、丙酮酸、乙醇、丙二醇和异丙醇。结论:金黄色葡萄球菌在生物被膜生长期间主要摄取了葡萄糖、精氨酸和苏氨酸,主要积累了2-氨基丁酸、鸟氨酸、乙酸、乳酸、乙醇和异丙醇。
Objective： To characterize metabolic profile of Staphylococcus aureus cultivated under biofilm condition,which will be useful for the development of novel identification and treatment tools. Methods： Nontargeted analyses of biofilm cultures were carried out using1 H nuclear magnetic resonance spectroscopy. Compounds were identified by Chenomx software. The changes of main metabolites were determined by using principal components analysis. Results ：41 compounds were identified in S. aureus biofilm cultures. S. aureus largely consumed 10 compounds during biofilm growth, including glucose, arginine, aspartate, threonine, acetoacetic acid, formate, succinic acid, glycerol, betaine and carnitine. Meanwhile, it accumulated 14 metabolites, including 1, 3-dihydroxyacetone, 2-aminobutyrate, ornithine, 3-hydroxyisovaleric acid, 3-methylglutaric acid, acetate, isobutyrate, isovalerate, lactate, pyroglutamate, pyruvate, ethanol,propylene glycol and isopropanol. Conclusion： S. aureus mainly consumed glucose, arginine and threonine during biofilm growth. Meanwhile, it mainly accumulated 2-aminobutyrate, ornithine, acetate, lactate, ethanol and isopropanol.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第12期258-264,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(20776153)
河北省自然科学基金项目(C2013204050)
关键词
金黄色葡萄球菌
代谢轮廓
生物被膜
1H核磁共振波谱法
Staphylococcus aureus
metabolic profile
biofilm
1H nuclear magnetic resonance spectroscopy
