摘要
目的了解上海市闵行区农贸市场食品中李斯特菌的污染情况及其毒力基因的携带情况。方法按国标方法GB/T4789.30—2008《食品卫生微生物学检验单核细胞增生李斯特氏菌检验》,采用科玛嘉显色培养基,对食品进行李斯特菌的分离、API试剂条进行生化鉴定,PCR扩增进行hly、prfA、plcB、actA、inlA和iap6种毒力基因的检测。结果320份样品中共检出李斯特菌36株,其中单核细胞增生李斯特菌10株;英诺克李斯特菌23株,其余3株。10株单核细胞增生李斯特菌hly、prfA、plcB、iap、inlA、actA6种毒力基因携带率分别为100%、100%、100%、100%、100%和0,即10株菌株actA基因全部缺失。结论本地区农贸市场食品存在李斯特菌的污染,生肉禽类食品中李斯特菌污染比较严重,应加强监督管理;单核细胞增生李斯特菌菌株actA毒力基因均表现为缺失,是否为本地区特性,还有待研究。
Objective To investigate the contamination of Listeria in some foods from farm markets and to detect the virulence genes of Listeria monocytogenes in Minhang district of Shanghai.Method Based on the national standard methods GB /T 4789.30—2008,Listeria in food was separated and biochemically identified by using CHROM agar color medium and API reagent strips.The virulence gene of hly,prfA,plcB,iap,inlA and actA were detect by PCR methods.Results There were 36 strains of Listeria isolated and identified from 320 samples,including 10 strains of Listeria monocytogenes,23 strains of Listeria innocua and 3 strains of other Listeria.Excepting the absence of actA,virulence genes hly,prfA,plcB,iap,inlA were identified in all 10 strains of Listeria monocytogenes.Conclusion The contamination of Listeria is existed in foods from farm market in Minhang district of Shanghai.Supervision and management should be strengthened on raw meat and poultry,which were serious contaminated.The absence of virulence gene actA in all Listeria monocytogenes isolated from local food needs further study.
出处
《中国食品卫生杂志》
北大核心
2010年第3期275-277,共3页
Chinese Journal of Food Hygiene
基金
上海市闵行区科委自然基金项目(2007MH005)
