摘要
目的采用PCR法扩增食源性金黄色葡萄球菌中肠毒素基因以了解该菌肠毒素基因携带情况,比较食物中毒和食品监测来源菌株中肠毒素基因检出率差异。方法合成sea、seb、sec、sed和see五种肠毒素基因特异性引物,用常规PCR方法扩增食物中毒和食品监测来源菌株中各自肠毒素基因,同时采用mini-VIDAS检测食物中毒来源菌株中肠毒素。结果 110株菌株中有30株检出肠毒素基因,检出率为27.3%,肠毒素基因阳性菌株均只检出1种肠毒素基因。其中来自2起食物中毒的14株菌株均检出seb型肠毒素和相关基因,检出率为100%。来源于食品监测样本的96株菌株中有16株检出肠毒素基因,检出率为16.7%,包括sea型4株、seb型2株、sec型4株、sed型6株。结论在宁波市食品监测中所分离的金黄色葡萄球菌所携带的肠毒素基因主要有sea、seb、sec和sed四型,而seb型肠毒素是引起金黄色葡萄球菌肠毒素所致食物中毒的主要因素。
Objective To study and compare the prevalence of enterotoxin genes in foodborne Staphylococcus aureus isolates from food-poisoning and food inspection. Methods Isolates from food-poisoning and food inspection were tested by PCR method with five pairs of primers for sea, seb, sec, sed and see genes. Enterotoxin was analyzed in isolates from food-poisoning isolates with mini-VIDAS. Results 30 out of 110 samples were enterotoxin gene positive （27.3% , 30/ 110） and only one type of enterotoxin gene existed in all positive samples. Both enterotoxin and relative gene of seb were found in 14 isolates from two food-poisoning events （100% , 14/14）. 16 isolates from 96 food inspection samples were enterotoxin gene positive （16.7% , 16/96） , including 4 strains of sea, 2 seb, 4 sec and 6 sed. Conclusion Enterotoxin genes of sea, seb, sec and sed were found in Staphylococcus aureus isolates in Ningbo. Enterotoxin of seb is the major factor of food-poisoning caused by Staphylococcus aureus.
出处
《中国食品卫生杂志》
北大核心
2012年第5期427-429,共3页
Chinese Journal of Food Hygiene
