摘要
分析了文冠果蛋白的氨基酸组成,并以文冠果压榨油渣为原料,采用单因素实验和正交实验优化了碱溶酸沉法提取文冠果蛋白的工艺条件。结果表明:文冠果蛋白含有18种氨基酸,其中谷氨酸(14.25%)、精氨酸(6.00%)和天门冬氨酸(5.08%)的含量较高;采用碱溶酸沉法提取文冠果蛋白的最佳工艺条件为料液比1∶30、提取pH 9、提取时间150 min、提取温度60℃,在此条件下提取2次,蛋白质得率为22.58%;酸沉分离蛋白质的最佳pH为4.6,此条件下蛋白质沉降率为94.67%;通过碱溶酸沉法可使文冠果蛋白的纯度从38.46%提高到84.03%。
The amino acid composition of Xanthoceras sorbifolia Bunge protein was analyzed. The alkali-solution and acid - precipitation （ ASAP ） processes for extracting protein from Xanthoceras sorbifolia Bunge oil residue were optimized by orthogonal experiment based on single factor experiment. The results showed that the Xanthoceras sorbifolia Bunge protein had eighteen kinds of amino acids, and the highest content of amino acid was glutamic acid （ 14 . 25%） , followed by arginine （ 6 . 00%） and asparaginic acid （5. 08%）; the optimal extraction conditions of Xanthoceras sorbifolia Bunge protein were obtained as follows：ratio of solid to liquid 1：30, extraction pH 9, extraction time 150 min, extraction temperature 60℃, the protein yield was 22 . 58% after extraction for twice under the optimal conditions;at the optimal acid-precipitation pH 4. 6,the protein sedimentation rate was 94. 67%; by ASAP extraction,the purity of Xanthoceras sorbifolia Bunge protein increased from 38 . 46% to 84 . 03%.
出处
《中国油脂》
CAS
CSCD
北大核心
2014年第7期42-45,共4页
China Oils and Fats
基金
国家自然科学基金(31360192)
甘肃省高等学校科研项目(2013B-009)
甘肃菁茂生态农业科技股份有限责任公司资助项目
关键词
文冠果
油渣
蛋白质
碱溶酸沉法
氨基酸组成
Xanthoceras sorbifolia Bunge
oil residue
protein
alkali-solution and acid-precipitation
amino acid composition
