摘要
建立了头发中色氨酸的荧光分析方法，用该法研究了UVB辐射下头发中色氨酸的变化规律。头发样品以5．5mol／L氢氧化钠溶液在110℃下水解20h，在PH10．5的磷酸二氢钠-氢氧化钠缓冲液中，激发波长为280nm、发射波长为360nm处测定色氨酸的荧光强度。方法线性范围为8．00×10^-8～1．75×10^6g／mL，检测限为1．6×10^-8g／mL，回收率在96．3％～100．3％。头发样品中色氨酸的量随UVB辐射时间增加呈减少趋势，光照28d后，色氨酸的损失率达到18．8％。该法快速、简便、灵敏度高。可作为衡量头发光损伤的定量指标。
A fluorescence spectrometry method has been developed to determine tryptophan in hair, and applied to evaluate hair damnification under UVB ultraviolet. 5.5 mol/L 1 NaOH solution containing 0.5% soluble starch was used for hydrolysis of hair samples. In buffer of NaH2PO4-NaOH（pH 10.5）, the fluorescent intensity of tryptophan was determined at 280nm as excitation wavelength and 360nm at emission wavelength. The linear range of tryptophan was from 8.0×10 ^-6 g/mL to 1.75× 10^-6g/mL. The detection limit of 1.6×10^-8g/mL and the recoveries ranged from 96.3% to 100.3%, these results showed that the method was sensitive and accurate. With increase of the irradiation time with UVB ultraviolet, tryptophan in hair was decreased. During 28days irradiation, tryptophan in hair was lost by 18.8%. Therefore this novel method could be used to measure ultraviolet damnification of hair.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2008年第4期30-33,共4页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(20775028)
关键词
荧光法
色氨酸
头发
光损伤
fluorescence spectrometry
tryptophan
hair
ultraviolet damnification
