摘要
目的:探讨我国宁夏人群中载脂蛋白E( ApoE)基因多态性与血清中尿酸水平的相关性。方法病例对照研究。2011年10月至11月,随机选取宁夏地区528名健康体检人群(男296名、女232名),采集空腹静脉血进行血生化检测,应用等位基因特异性多重PCR(multi-ARMS PCR)技术,对ApoE基因进行分型,分析基因型与血清血脂水平和尿酸水平的关系。非正态分布者比较采用致和检验。结果 ApoE常见的6种基因型均可检出,不同ApoE基因型的总胆固醇( TC)、低密度脂蛋白胆固醇( LDL-C )和尿酸( UA )水平差异有统计学意义。 ApoE ε2/3基因型的 TC [(3.50±0.83)mmol/L]、LDL-C[(1.87±0.60) mmol/L]水平低于ε3/3[(3.85±0.84)mmol/L、(2.24±0.69) mmol/L]和ε3/4[(3.89±0.78) mmol/L、(2.34±0.68) mmol/L]基因型,差异有统计学意义,F=5.619,P<0.05。 UA水平[(374.9±97.8) mmol/L]则显著高于ε3/3[(328.4±105.5) mmol/L]和ε3/4[(339.2±98.3) mmol/L],差异有统计学意义,F=6.052,P<0.05。进一步对年龄、性别、地区等因素进行校正后ApoE基因多态性对UA水平的影响依然显著。结论 ApoE基因多态性与血清尿酸水平相关,含ε2等位基因者具有更高的血清尿酸水平。(中华检验医学杂志,2014,37:434-438)
Objective Toexplore the correlation of ApoE gene polymorphism and serum uric acid levels in Ningxia Hui Autonomous Region , China.Methods A case-control study.October 2011 to November 2011, five hundred twenty eight ( 296 male, 232 female ) apparently healthy individuals were studied.Questionnaires and physical examinations were performed by standard operation procedure.Fasting blood was collected for biochemistry testing including serum lipid parameters , uric acid concentration and creatinine levels.The multi-ARMS PCR was applied to determine ApoE genotypes ,and the relation of ApoE genotypes with serum lipid parameters and uric acid levels were analyzed.Non-normal distribution were compared using cause and inspection.Results The common six kinds of ApoE genotype can be detected.The total cholesterol ( TC) ,low density lipoprotein cholesterol ( LDL-C) and uric acid ( UA) levels in different genotype subgroups had statistical differences.The individuals with ε2/3 genotype had a significantly greater reductions in TC and LDL-C levels but increment in uric acid concentration than those withε3/3 and ε3/4 genotype (P〈0.05).The effect of ApoE gene polymorphism on uric acid levels still remained significantly after adjustment for age , gender , region and other factors.Conclusion The ApoE polymorphism is associated with serum uric acid levels and individuals with ε2 allele have higher serum uric acid levels.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2014年第6期434-438,共5页
Chinese Journal of Laboratory Medicine
基金
国家科技部重点专科建设项目资助(2006FY110300)
关键词
载脂蛋白E类
多态现象
遗传
尿酸
脂类代谢
Apolipoproteins E
Polymorphism,genetic
Uric acid
Lipid metabolism