摘要
目的探讨载脂蛋白E（apoE）基因多态性与脑梗死的关系。方法应用聚合酶链式反应-限制性片断长度多态性（PCR—RFLP）技术检测78例脑梗死患者和90名健康对照者apoE基因多态性分布特征。结果发现5种apoE基因型，脑梗死组E3／4基因型频率（23．1％）及ε4频率（14.7%）显著高于对照组（7．8％，5．0％），P〈0．05。而E3／3基因型频率（56．4％）及ε3频率（75.6%）显著低于对照组（78．9％，88．3％），P〈0．05。不同apoE基因型间胆固醇和低密度脂蛋白胆固醇水平差异有统计学意义（P〈0．05）。结论apoE基因多态性与脑梗死有关，并影响血脂水平。
Objective To investigate the association between apoliporotein E gene polymorphism and cerebral infarction. Methods The polymerase chain reaction （PCR） and restriction fragment length polymorphism （RFLP） technique were used to detect apoE geno in 78 cerebral infarction（CI） patients and 90 age-matched controls. Results There were five kinds of apoE genotypes. The frequency of genotype E3/4 was 23.1% and ε4 was 14.7% in the CI group, which were significantly higher than in the control group （P〈0.05）, while the frequency of genotype E3/3 was 56.4 % and ε3 was 75.6 % in the CI group, which was significantly lower than in the control group（P〈0.05）. The serum levels of total cholesterol （TC） and low density lipoprotein cholesterol （LDL-C） were significantly different among different apoE genotype subgroups（P〈0.05）. Conclusions The polymorphisms of apolipoprotein E gene are associated with CI and the serum levels of TC and LDL-C.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2007年第7期498-500,共3页
Chinese Journal of Geriatrics
关键词
载脂蛋白E类
多态现象
遗传
脑梗死
Apolipoproteins E
Polymorphism, genetic
Brain infarction
