摘要
本研究对４２例皮质脑梗塞患者及８５名对照者采用ＰＣＲ－ＡＦＬＰ法检测载脂蛋白Ｅ（ａｐｏＥ）基因型及ＥＬＩＳＡ法检测血脂蛋白（ａ）［Ｌｐ（ａ）］水平。结果显示各ａｐｏＥ基因型频率，病人组以ε３／４多见（５７．１４％），而对照组以ε３／３型多见（５５．２９％）；病人组ε４等位基因频率显著高于对照组（Ｐ＜０．０５），ε３／４基因型及ε４等位基因对皮质脑梗塞发病危险度比数比分别为２．４４和２．３１。各ａｐｏＥ基因型间的Ｌｐ（ａ）水平并无明显差异。病人组各ａｐｏＥ基因型血ＴＧ和ＴＣ水平明显差别。本研究提示ａｐｏＥε４等位基因是易感脑梗塞的遗传因素，支持ａｐｏＥ多态性与ＡＳ性疾病相关的理论假说；同时还得出Ｌｐ（ａ）水平变化不受ａｐｏＥ多态性影响。
In this study, we measured the apoE genotypes by means of PCR-AFLP assay and the serum lipoprotein(a) by ELISA way in 42 patients with cortical cerebral infarction and 85 healthy controls. The results showed that ε 3/4 genotype was more common in the patients (57.14%), and the apoE ε 3/3 genotype was more prevalent in the controls (55.29%). The frequency of ε 4 allele was more predominate in the patients than in the controls(P<0.05). The ε 3/4 genotype or ε 4 allele was associated with 2.44 or 2.31 fold increase in the risk of cortical cerebral infarction. The serum lipoprotein(a) levels among the subjects with different apoE genotypes were not changed significantly.There was obvious difference in the levels of TG amd TC with different apoE genotypes in the patients group.Therefore, it was suggested that apoEε4 allele is a genetic factor of susceptibility to cerebral infarction, which supported the hypothesis of the association of apoE with atherosclerotic cardio-cerebrovascular disorders. The polymorphism of apoE gene probably have no role in the metabolism of lipoprotein(a).
出处
《临床神经病学杂志》
CAS
1998年第3期138-141,共4页
Journal of Clinical Neurology
关键词
载脂蛋白E
基因多态性
脑梗塞
病理
Apolipoprotein E Gene Polymorphism Cerebral infarction
