摘要
目的 :探讨载脂蛋白 E(Apo E)基因多态性与冠心病 (CHD)及脑梗塞 (ACI)的关系。方法 :以聚合酶链反应-限制性片段长度多态性技术 (PCR- RFL P)结合银染色方法检测了 6 3例 CHD、4 0例 ACI患者和 90例无血缘关系的健康汉族人群 Apo E基因型。结果 :CHD组和 ACI组的 ε4 等位基因频率均明显高于对照组 (P<0 .0 5 ,P<0 .0 1) ,而 ε3等位基因频率均明显低于对照组 (P<0 .0 5 ,P<0 .0 1) ,ε4 和 ε3等位基因频率在 CHD组和 ACI组间无差别 (P>0 .0 5 )。结论 :ε4 等位基因是冠心病和脑梗塞的遗传易患因子之一 ,ε3等位基因是冠心病和脑梗塞的保护因子。
Objective:To study the relationship of gene polymorphism of Apolipoprotein E(ApoE) with coronary heart disease(CHD) and atherosclerotic cerebral infarction (ACI).Methods:The ApoE genotypes of 63 CHD patients, 40 ACI patients and 90 cases of unrelated normal Chinese were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)combining silver staining.Results:The frequencies of ε 4 allele in CHD and ACI patients were significantly higher than that in the control group(P<0.05,P<0.01 respectively),while frequencies of ε 3 allele were lower(P<0.05,P<0.01 respectively).There is no difference of the frequencies of ε 3 and ε 4 allele uin CHD group and ACI group.Conclusion:ApoE ε 4 allele is probably the genetic marker of ACI and CHD, while ε 3 allele is a protective factor.
出处
《中国误诊学杂志》
CAS
2004年第1期19-21,共3页
Chinese Journal of Misdiagnostics
