摘要
采用离体花粉液体培养法研究4种植物生长调节物质对不同来源楸树花粉萌发及花粉管生长的影响。结果表明:较低浓度的赤霉素促进花粉萌发及花粉管生长,高浓度则表现为明显的抑制作用,最适浓度为300 mg/L;吲哚乙酸和2,4-D对花粉的作用效果与楸树的种源有关,总体上均呈低浓度促进,高浓度抑制的规律;花粉萌发率均在吲哚乙酸为15 mg/L时达到最大值,只有2个种源与对照间差异极显著,花粉萌发率在2,4-D为200 mg/L时均达到最大值,超过最适浓度后,吲哚乙酸和2,4-D均对花粉萌发及花粉管生长表现为抑制作用;各个浓度的萘乙酸均抑制花粉萌发及花粉管生长,且抑制程度随浓度增加而加强。
The effect of four types of plant growth regulating substances on pollen germination and pollen tube growth of different Catalpa bungei provenances was studied by in-vitro pollen cultivation in liquid media.The re-sults showed that low concentration of GA3 stimulated pollen germination and pollen tube growth,whereas the high concentration of GA3 had inhibition effect.The most suitable concentration to stimulate the pollen germination and pollen tube growth was 300 mg/L.The effect of IAA and 2,4-D varied with the C.bungei provenances,but the general law was that the low concentrations could promote but the high concentrations would inhibit the pollen ger-mination and pollen tube growth.The maximum pollen germination rate was obtained by 15 mg/L IAA concentra-tion,there was significant difference in the pollen germination rate among two provenances and the control.Regard-ing to 2,4-D,the maximum pollen germination rate was obtained by 200 mg/L concentration.When the concen-trations of both IAA and 2,4 -D were over the most suitable values,these two types of plant growth regulators would inhibit the pollen germination and pollen tube growth.NAA had an inhibition effect on the pollen germination and pollen tube growth of C.bungei at different concentrations,the higher NAA concentration was,the sronger the inhibition effect would be.
出处
《西南林业大学学报（自然科学）》
CAS
2014年第2期13-18,共6页
Journal of Southwest Forestry University：Natural Sciences
基金
国家"十二五"科技支撑计划项目子课题(2012BAD21B03)资助
江苏省高技术项目(BG2006319)资助
江苏省普通高校研究生科研创新基金资助
关键词
楸树
植物生长调节物质
花粉萌发率
花粉管生长
Catalpa bungei
plant growth regulators
pollen germination rate
pollen tube growth
