摘要
为给红锥(Castanopsis hystirx)人工授粉和新品种培育提供基础,测定红锥花粉萌发率和花粉管长度,探讨花粉的最佳离体萌发条件和贮藏条件。结果表明,采用100 g/L蔗糖+200 mg/L硼酸培养液,培养温度为25℃,花粉萌发率最高(28.23%),花粉管长度最长(260.45μm);采用-20℃+干燥剂或-80℃+干燥剂贮藏花粉效果较好,贮藏365天,萌发率仍有30.54%和26.43%,花粉管长度为108.61和95.65μm。低温、干燥有利于红锥花粉贮藏。
In order to provide a basis for artificial pollination and new variety breeding of Castanopsis hystrix,Germination rate and pollen tube length of C.hystrix pollen were measured to explore optimal condition for pollen germination in vitro and storage condition.Results showed that the highest pollen germination rate(28.23%)and the longest pollen tube length(260.45μm)were achieved by using 100 g/L sucrose+200 mg/L boric acid culture solution and incubation temperature of 25℃.Pollen storage at-20℃+desiccant or-80℃+desiccant was more effective,with 30.54%and 26.43%germination rates and 108.61 and 95.65μm pollen tube lengths after 365 d.Low temperature and dryness were favorable for pollen storage of C.hystrix.
作者
郝海坤
申文辉
曹艳云
黄志玲
谭长强
Hao Haikun;Shen Wenhui;Cao Yanyun;Huang Zhiling;Tan Zhangqiang(Guangxi Forestry Research Institute,Key Laboratory of Central South Fast-growing Timber Cultivation of National Forestry and Grassland Administration,Nanning,Guangxi 530002,China)
出处
《广西林业科学》
2022年第2期217-222,共6页
Guangxi Forestry Science
基金
中央引导地方科技发展资金项目(桂科ZY21195049)
广西优良用材林资源培育重点实验室自主课题(2020-A-02-02)。
关键词
贮藏方法
花粉离体萌发
红锥
萌发率
storage method
in vitro pollen germination
Castanopsis hystrix
germination rate
