摘要
【目的】探讨培养基中的钙、镁、钾浓度及光照、温度对西番莲花粉体外萌发及花粉管生长的影响。【方法】设置钙、镁、钾及光照、温度梯度,测量在该梯度下的花粉萌发率及花粉管延伸长度。【结果】钙、镁、钾非西番莲花粉离体萌发的必需培养基成分,钙对西番莲花粉离体萌发及花粉管生长具有显著影响,在质量浓度为0~300 mg·L^(-1)时,萌发率及花粉管长度均随浓度的增加而显著增加,在300~1200 mg·L^(-1)时最佳,大于1200 mg·L^(-1)时,萌发率及花粉管长度均显著降低。镁、钾对花粉萌发及花粉管生长均无显著影响。光照、温度对花粉萌发及花粉管生长均具有显著影响,黑暗条件下最佳,随着光照度的增加,萌发率及花粉管长度均显著降低;二次方程能较好地拟合温度对花粉萌发及花粉管生长的影响。【结论】培养基中钙浓度及光照、温度对西番莲花粉体外萌发及花粉管生长具有显著影响,镁、钾无显著影响,最适的Ca(NO_(3))_(2)·4H_(2)O质量浓度为300~1200 mg·L^(-1),西番莲花粉离体萌发的最适培养基为150 g·L^(-1)蔗糖+150 g·L^(-1) PEG-4000+25 mg·L^(-1) H_(3)BO_(3)+300~1200 mg·L^(-1) Ca(NO_(3))_(2)·4H_(2)O,在该培养基组分下的花粉萌发率为86%以上。0 lx(黑暗)、25.69℃分别为西番莲花粉萌发及花粉管生长的最适光照及温度,温度对西番莲花粉萌发及花粉管生长的影响符合二次项方程。
【Objective】Pollen germination in vitro is an important means to detect pollen activity.So far,the effect of calcium,magnesium,and potassium concentrations in the medium,and light and temperature conditions on Passiflora pollen germination and tube elongation are not yet clear,and therefore,the research on these influence factors not only leads to further optimizing the germination conditions,but also provides theoretical guidance for the Passiflora cultivation.【Methods】The experiment was conducted from September to November,2020 in the Experimental Station(N 22.85°,E 108.24°),Horticulture Institute,Academy of Agricultural Sciences in Guangxi Zhuang Autonomous Region.The maximum temperature during the period was around 37℃,and the minimum temperature was 20℃.The fresh pollen of Passiflora caerulea L.‘Tainong No.1’as a China’s main variety was used as the experimental material,the flowers were picked up when they were just opened,and two fully-bloomed flowers were collected from each of the 10 plants.The flowers were put into paper bags and brough to the laboratory immediately.The anther was gently removed in the laboratory with air conditioner of 25℃,and placed in a petri dish lined with weighing paper,with dried silica gel under the paper.The petri dish was placed in a ventilated and dry place for about 2 h,until the anthers were fully cracked,and then the pollen grain was swept down gently with a soft brush,mixed with all the pollen for later use.The medium consisted of 150 g·L^(-1) sugar,150 g·L^(-1) PEG-4000,25 mg·L^(-1) H_(3)BO_(3),300 mg·L^(-1) Ca(NO_(3))_(2)·4H_(2)O,200 mg·L^(-1) MgSO_(4)·7 H_(2)O,and 100 mg·L^(-1) KNO_(3) was used as a basic medium.0,100,300,600,1200,2400 mg·L^(-1) of Ca(NO_(3))_(2)·4H_(2)O and 0,200,400,800,1600,3200 mg·L^(-1) of MgSO_(4)·7 H_(2)O and 0,100,200,400,800 and 1600 mg·L^(-1) of KNO_(3) were set to study the effect of calcium,magnesium and potassium concentrations on pollen germination in vitro under 25℃and dark condition.0 lx(dark),600 lx,
作者
蔡昭艳
苏伟强
董龙
邱文武
施平丽
刘业强
黄辉晔
黄章保
任惠
王小媚
CAI Zhaoyan;SU Weiqiang;DONG Long;QIU Wenwu;SHI Pingli;LIU Yeqiang;HUANG Huiye;HUANG Zhangbao;REN Hui;WANG Xiaomei(Institute of Horticultural,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;Institute of Plant Protection,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China)
出处
《果树学报》
CAS
CSCD
北大核心
2022年第1期86-94,共9页
Journal of Fruit Science
基金
广西农业科学院基本科研业务费专项(桂农科2020YM30)
广西农业科学院科技发展基金项目(桂农科2021JM10)
国家现代农业产业技术体系广西特色水果创新团队南宁百香果试验站(nycytxgxcxtd-17-02)
广西农业科学院基本科研业务费专项(桂农科2021YT046)。
关键词
西番莲
花粉
离体萌发
培养基
光照
温度
Passiflora
Pollen
In vitro germination
Medium
Light
Temperature
