摘要
目的研究易层贴敷疗法对骨性关节炎(OA)中滑膜炎症因子及基质金属蛋白酶表达的影响。方法 30只新西兰大白兔,随机分为正常组、模型对照组及治疗组;模型对照组及治疗组通过切断兔左后肢前交叉韧带获得OA模型,术后6周外用易层贴敷;术后8周处死动物,取滑膜行Western Blot及荧光定量PCR检测IL-1β、MMP-1及MMP-13的含量及表达。结果 Western Blot测定模型对照组滑膜中MMP-1及MMP-13含量明显高于治疗组,正常组中含量最低;荧光定量PCR测定模型对照组中MMP-1及MMP-13的表达量最高,正常组最低;正常组滑膜中IL-1β表达明显最低,模型对照组与治疗组之间无统计学差异。结论易层贴敷疗法能有效抑制滑膜中MMP-1及MMP-13的表达,从而降低了软骨基质的降解,缓解OA的进程,仍需进一步研究其具体的作用靶点,完善其作用机制。
OBJECTIVE To investigate the effect of Layers Adjusting External Application Therapy on the expression of synovial inflammatory factors and matrix metalloproteinase （MMPs） in osteoarthritis （OA）.METHODS 30 New Zealand rabbits were randomly divided into normal group,control group and treatment group.OA model was induced in rabbit by unilateral posterior anterior cruciate ligament transection （ACLT）.6 weeks post-operation,the Layers Adjusting External Application Therapy were used externally; 8 weeks post-operation,the rabbits were sacrificed.The synovial membrane specimens were taken to detect the expression and content of IL-1β,MMP-1 and MMP-13 by Western Blot and Fluorogenic Quantitative PCR.RESULTS Western Blot：The content of MMP-1 and MMP-13 in the control group were significantly higher than the treatment group.The content of MMP-1 and MMP-1 in the normal group were the lowest Fluorogenic Quantitative PCR.The expression of MMP-1 and MMP-13 in the control group were the highest the expression of MMP-1 and MMP-13 in the normal group were the lowest the expression of IL-1β in the normal group was significantly lowest.There were no significantly differences in the expression of IL-1β between control group and treatment group.CONCLUSION Layers Adjusting External Application Therapy can effectively inhibit the expression of MMP-1 and MMP-13 in the Synovial Membrane,thus reduces the degradation of cartilage matrix alleviate the progression of OA.Nevertheless,further research are needed to investigate the specific target so that the mechanism of the therapy can be revealed.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2014年第2期134-137,共4页
Journal of Nanjing University of Traditional Chinese Medicine
关键词
易层贴敷疗法
骨性关节炎
滑膜
基质金属蛋白酶
layers adjusting external application therapy
osteoarthritis
synovial membrane
matrix metalioproteinases
