摘要
目的建立超高效液相色谱(UPLC)同时测定茶叶中没食子酸(GA)、茶碱(THEO)、咖啡因(CAFF)、没食子儿茶素(GC)、儿茶素(C)、表儿茶素(EC)、表没食子儿茶素(EGC)、表没食子儿茶素没食子酸酯(EGCG)、没食子儿茶素没食子酸酯(GCG)、表儿茶素没食子酸酯(ECG)的分析方法。方法以甲醇-水-磷酸为流动相,BEH C18分离,梯度洗脱,230 nm波长检测,结果 10种组分的线性范围为:0.1 mg/L～20 mg/L,回收率在94.9%～102.3%,RSD为0.7%～4.09%。结论方法简便、重现性好,灵敏度高。
Objective To establish a method for simultaneous determination of gallic acid （GA）, theophyline （THEO）, caffe-ine（ CAFF）, gallocatechin （ GC ）, catechin （ C ）, epicatechin （ EC ）, epigalloeatechin （ EGC ）, epigallocatechin - 3 - gallate （ EGCG）, gallocatechin gallate （ GCG）, epicatechin - gallate （ECG） in tea by UPLC. Methods The tea extract was separa ted on the BEH C18 column, eluted with methanol - water - phosphoric acid for gradient elution, and detected by UPLC at wavelength of 230nm. Results The linearity of the method was in the range of 0.1 mg/L - 20 rag/L, the recovery rates were 94.9%- 102.3% with RSDs of 0.7% - 4.09%. Conclusion This method is convenient, precise and reproducible. It is suitable for simultaneous determination of aforementioned components in tea.
出处
《中国卫生检验杂志》
北大核心
2013年第16期3167-3169,共3页
Chinese Journal of Health Laboratory Technology
基金
深圳出入境检验检疫局科研项目(SZ2009017)
国家质检总局科研项目(2008IK119)
关键词
超高效液相色谱(UPLC)
儿茶素
酚酸
生物碱
茶叶
Ultra performance liquid chromatography（UPLC）
Catechins
Phenolic acid
Alkaloids
Tea
