摘要
研究了OPA柱前自动衍生-紫外检测茶汁中茶氨酸的 HPLC法,色谱条件为:Hypeml ODS C18柱,洗脱液为醋 酸钠和甲醇混合液,采用梯度洗脱,流速为1.0mL／min. 检测波长为338nm。茶氨酸在0.05-1.00mg／mL范围内浓 度与峰面积线性关系良好,线性相关系数为0.9998,平均 回收率为98.85％,RSD小于1％(n=5)。
Determination of theanine by HPLC with automatic pre -column derivation and UV detection was studied. Chromatographic conditions are as following: Hypersil ODS C18 column, eluant is gradient mixture of water solution of 20mmol sodium acetate and 20mmol methanol, flow rate 1.0mL/min, temperature of column 40℃and UV detection wavelength of 338 nm. There was a good line correlation between peak area and contents in the range of 0.05-1.00mg/mL, the correlation coefficient was 0.9998, the average recovery was 98.85% with a relative stand deviation of 0.54%(n=5).
出处
《食品工业科技》
CAS
CSCD
北大核心
2005年第2期182-183,共2页
Science and Technology of Food Industry
