摘要
利用组织培养技术结合高效液相色谱法,考察了液体悬浮培养中无机盐和蔗糖浓度对白色紫锥菊不定根生长以及紫锥菊苷、菊苣酸、氯原酸和酚类化合物积累的影响。结果表明:白色紫锥菊不定根在高或低浓度无机盐和蔗糖培养基中的生长及次生代谢产物含量均较低,不定根生长最适培养基为0.75 MS+5%蔗糖,培养30 d后不定根中紫锥菊苷含量为7.40 mg/g DW,菊苣酸为3.96 mg/g DW,氯原酸含量达3.79 mg/g DW,总酚含量达25.62 mg/g DW。本研究为进一步大规模培养富含紫锥菊苷、菊苣酸的白色紫锥菊不定根奠定了理论和实践基础。
In this study, the effects of medium salt strength and initial sucrose concentration on biomass increase and the accumulation of echinacoside, ehichoric acid, ehlorogenic acid and total phenolics in suspension cultures of Echinacea pallida adventitious roots were investigated. Secondary metabolites were analyzed by high performance liquid chromatog- raphy （HPLC） and spectrophotometer. 0.75 MS medium supplemented 5% （w/v） sucrose resulted in the optimum ac- cumulation of hiomass and secondary metabolites, whereas lowest/highest salt strengths and sucrose concentrations all in- hibited root growth and metabolite accumulation. Under optimal culture conditions 7.40 mg/g dry weight （DW） of echi- nacoside ,3.96 mg/g DW of chichoric acid ,3.79 mg/g DW of ckloregenie acid ,25.62 mg/g DW of total phenollcs were produced in the adventitious roots after 30 days of culture. The experimental results contributed to the development of bioreaetor technology for adventitious root cultures of E. paUida for the production of caffeic acid derivatives.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第9期1167-1171,共5页
Natural Product Research and Development
基金
大连市科技计划项目(2009E11SF126)
大连市科学技术基金留学回国人员项目(2009J21DW001)
关键词
白色紫锥菊
不定根
次生代谢产物
紫锥菊苷
培养基浓度
Echinacea paUida
adventitious roots
secondary metabolltes
echinacoside
medium salt strength
