摘要
目的建立高效液相色谱法测定杜仲愈伤组织和悬浮细胞中绿原酸含量。方法利用Hypersil C18-ODS柱（250mm×4．6mm，5μm）色谱柱，乙腈-水-磷酸溶液（12：87．9：0．1）为流动相，检测波长为327nm，流速1．0ml／min，柱温为25％。结果绿原酸在2．216～55．4μg/ml范围内线性关系良好，r=0．9981，愈伤组织和悬浮细胞中绿原酸的平均加样回收率分别为100．69％和97．62％，RSD分别为2．42％和2．71％。结论该方法快速简便，准确可靠。杜仲愈伤组织和悬浮细胞中绿原酸含量分别为1．528mg／g和3．949mg／g。
Objective To establish an HPLC method for the determination of chlorogenic acid in callus and suspension ceils of Eucommia ulmoides Oliv. Methods The separation was performed on Hypersil C18 - ODS （250mm × 4.6mm,5 μm） column, using acetonitrile - water - H3PO4 （ 12： 87.9： 0.1 ） as mobile phase, detected at 327 nm. The flow rate was 1.0 ml/min, the column temperature was 25 ℃. Results The linear range of chlorogenic acid was 2. 216 - 55.4 mg/ml, r = 0. 998 4, the average recoveiy of chlorogenic acid in casllus and suspension cells were 100.69% and 97.62% , RSD were 2.42% and 2.72% , respectively. Conclusion The method is rapid, simple, accurate and stable. Chlorogenic acid content in callus and suspension cells of Eucommia ulmoides 01iv are 1. 528 mg/g and 3. 949mg/g respectively.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第9期2082-2083,共2页
Lishizhen Medicine and Materia Medica Research
基金
教育部重点科技资助项目(No.地方204078)
