摘要
目的:比较杜仲愈伤组织和悬浮细胞生长及其次生代谢产物黄酮和绿原酸的积累。方法:将杜仲无菌苗真叶诱导的愈伤组织进行悬浮培养,研究了悬浮培养物生长的动态和总黄酮类及绿原酸产量积累的动态。结果:杜仲愈伤组织中黄酮和绿原酸最高分别达到13.46,1.712 mg.g-1,而悬浮细胞中黄酮和绿原酸最高分别达到16.63,3.93 mg.g-1。结论:与愈伤组织相比,悬浮细胞具有生长周期短、速率快、黄酮和绿原酸积累量高的优势。
Objective： To analyze and compare the cell growth and accumulation of flavonoids and chlorogenic acid in the callus and suspension cell of Eucommia ulmoides. Method： The callus induced from the leaf of E. ulmoides seedlings were suspended in liquid medium. The time courses of cell growth and yields of flavonoids and chlorogenic acid were studied. Result： The highest contents of flavonoids and chlorogenic acid in the callus were 13.46, 1. 712 mg·g^-1, respectively, while the contents of these two secondary metabolites were 16.63, 3.93mg·g^-1 in suspension cell culture correspondingly. Conclusion： Comparing with callus, the suspension cell showed a short growth period and high growth rate with a remarkable high content of flavonoids and chlorogenic acid.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第13期1636-1639,共4页
China Journal of Chinese Materia Medica
基金
教育部重点科技项目(地方204078)
关键词
杜仲
愈伤组织
悬浮细胞
黄酮
绿原酸
Eucommia ulmoides
callus
suspension cell
flavonoids
chlorogenic acid
