摘要
目的评价全反式维甲酸逆转兔颊黏膜鳞状上皮癌前病变的效果。方法在已制备的兔鳞状上皮癌前病变模型上,贴附全反式维甲酸(ATRA)药膜,分别采用病理组织学、免疫组织化学及流式细胞术观察病理变化、癌基因及抑癌基因蛋白的表达,以及细胞的凋亡及增殖情况。结果 12周后,对照组16只家兔中2只逆转,6只病情稳定,8只恶化;ATRA组16只家兔中7只逆转,7只稳定,2只恶化;免疫组织化学显示,随ATRA逆转实验进行,增殖细胞核抗原(PCNA)、Bcl-2蛋白表达下降,P53蛋白表达上升,凋亡率上升,增殖指数降低。结论 ATRA有望成为有效的颊黏膜鳞状上皮癌前病变阻断药物。
Objective To evaluate the effect of all-trans-retinoicacid（ATRA） in the reversion of rabbit buccal mucosa precancerous lesion.Methods The established model of rabbit buccal mucosa precancerous lesions was exposed to the membrane containing ATRA.The pathological changes of buccal mucosa exposed to ATRA were observed by histopathology,immunohistochemistry and flow cytometry.Results Twelve weeks later,only 2 rabbits of 16 rabbits in the blank control group were reversed,6 rabbits stabilized and 8 rabbits progressed.Among 16 rabbits in the ATRA group,7 rabbits were reversed,7 rabbits were stable and 2 rabbits were worsen.The immunohistochemistry results showed that the positive rates of PCNA and bcl-2 protein expression were decreased as precancerous lesion reversing.P53 protein expression was increased,the apoptosis rate risen and the proliferation index was decreased.Conclusion ATRA is hopeful to become an effective blocking drug for buccal mucosa squamous epithelium precancerous lesion.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第13期1493-1494,1498,共3页
Chongqing medicine
基金
河北省卫生厅2010年医学科学研究重点课题计划基金资助项目(20100432)
