摘要
目的:建立一种可靠的肿瘤细胞分化模型,确立一套简便、准确判定肿瘤细胞是否分化的方法。方法:以分化诱导剂全反式维A酸(ATRA)影响下不同时间点的HL-6 0细胞为检测对象,流式细胞术分析细胞大小及细胞表面的分化标志物;经碘化丙锭染色后,用激光共聚焦显微镜观察对已分化细胞进行形态学确认。结果:随着药物诱导时间的延长,被诱导的HL-6 0细胞体积逐渐增大;72h后,被ATRA诱导细胞开始表达分化标志物CD11b并出现细胞核型的变化。结论:ATRA能诱导HL-6 0细胞分化;流式细胞术分析细胞大小及细胞表面的分化标志物,再用激光共聚焦显微镜观察已分化细胞核的形态变化是判定肿瘤细胞分化简便。
Objective:To establish a constant model of malignant tumor cell differentiation and to set up a set of methods that the differentiated cells can be identified.Methods:HL-60 cells influenced by all-trans retinoic acid(ATRA) capable of inducing differentiation at different time were used as subjects.The differentiation marker on the cell surface and the cell volume were analyzed by using flow cytometry.The differentiated cells were identified by confocal microscope after having been stained with PI.Results:With the drug-inducing time increasing,the volume of differentiated cells was enlarged gradually.After 72 hours,the differentiated cells induced by ATRA began to express differentiating marker CD11b and the differentiated cells,nuclei was changed into the nucleus morph differentiation just like nutrophil nuclei.Conclusions:The differentiation of HL-60 cells can be induced by ATRA.HL-60 cells,differentiation can be conveniently judged by combining the cell svolume,and the cells differentiating marker,analyzed by flow cytometry with morphodifferentiation observed by confocal microscope after having been stained with propidium iodide.
出处
《蚌埠医学院学报》
CAS
2005年第3期189-192,共4页
Journal of Bengbu Medical College
基金
国家自然科学基金 (No .3 973 0 2 70 )
国家杰出青年基金(No.3 972 5 0 2 7)
973肿瘤计划 (G19980 5 12 13 )
卫生部临床重点学科基金资助项目 (No .2 0 0 12 5 3 7)
