摘要
目的研究全反式维甲酸(ATRA)对人胃癌细胞增殖和细胞凋亡的影响及其可能的机制。方法用不同浓度的全反式维甲酸处理人胃癌细胞AGS,采用MTT法观察处理前后细胞增殖的变化,应用流式细胞仪和Western印迹杂交法检测细胞凋亡。结果 ATRA显著抑制胃癌细胞增殖,具有时间和浓度依赖性。在0.5、1.0和5.0μmol/L浓度ATRA作用下,胃癌细胞增殖明显受到抑制,且随着作用时间的延长和浓度的增加,抑制作用越明显。ATRA 5.0μmol/L处理细胞72 h后,胃癌细胞凋亡率为15.32%,而对照组为3.31%,差异有统计学意义(P<0.01);并且ATRA处理后可观察到聚二磷酸腺苷核糖多聚酶(PARP)的剪切降解,以及半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-3,-8和-9的激活。结论全反式维甲酸可抑制胃癌细胞的增殖,并诱导胃癌细胞凋亡,其机制与Caspases的活化有关。全反式维甲酸在胃癌的治疗中可能具有潜在的应用前景。
Objective To investigate the effect of all-trans retinoic acid（ATRA）on proliferation and apoptosis of human gastric carcinoma cell line AGS.Methods AGS cells were treated with different concentrations of ATRA.Cell proliferation was measured by MTT assay.Flow cytometry and Western blot were employed to detect the apoptosis of AGS cells.Results ATRA significantly inhibited the proliferation of AGS cells in a time-and dose-dependent manner.After treatment with different concentrations of ATRA（0.5,1.0 and 5.0 μmol/L）,the proliferation of AGS cells were inhibited significantly.The apoptosis rate of AGS cells treated with 5.0 μmol/L ATRA for 72 h was significantly higher（15.32%）than that of control cells（3.31%,P〈0.01）.Poly（ADP-ribose）polymerase cleavage was observed in AGS cells treated with ATRA,but not in control cells.Caspase-3,Caspase-8,and Caspase-9 activation was observed after ATRA treatment in AGS cells.Conclusion ATRA can inhibit the proliferation of AGS cells and induce apoptosis,and this retinoic acid-induced apoptosis may be Caspase-dependent.Retinoic acid is a potential therapeutic agent for gastric cancer.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2011年第3期301-303,319,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
湖北省自然科学基金资助项目(No.2006ABA103)
关键词
维甲酸
胃癌
细胞增殖
细胞凋亡
retinoic acid
gastric carcinoma
cell proliferation
apoptosis
