摘要
目的:研究全反式维甲酸(all-trans retinoic acid,ATRA)对胃癌细胞SGC-7901存活率与放射敏感性的影响,并讨论其可能的机制。方法:MTT法检测细胞存活率;平板克隆形成实验和流式细胞术分别检测细胞的放射敏感性和细胞周期;实时荧光定量PCR(RT-q PCR)检测细胞中Bax、Bcl-2、survivin与NF-κB的m RNA表达。结果:ATRA可降低SGC-7901细胞存活率,当浓度到达8μmol/L时,抑制作用达到最大;ATRA联合X射线处理后,与单纯放射处理相比,平均致死剂量(D0)和准阈剂量(Dq)显著变小(P<0.05),且拟合的生存曲线明显下移;ATRA能显著降低放射诱导的细胞G_2/M期阻滞,下调SGC-7901细胞Bcl-2与survivin的m RNA表达(P<0.05),上调Bax与NF-κB的m RNA表达(P<0.05)。结论:ATRA能够增加胃癌细胞SGC-7901的凋亡及放射敏感性,可能与抑制细胞周期G_2/M期的阻滞作用、下调Bcl-2与survivin m RNA表达和上调NF-κB与Bax mRNA表达有关。
AIM： To investigate the effect of all-trans retinoic acid（ATRA） on the viability of gastric cancer cell SGC-7901 and the sensitivity to radiotherapy. METHODS： MTT assay was used to examine the cell viability. Radiosensitivity and cell cycle were determined by colony formation assay and flow cytometry,respectively. The m RNA levels of Bax,Bcl-2,survivin and NF-κB in the cells were measured by RT-q PCR. RESULTS： ATRA inhibited the viability of SGC-7901 cells in a concentration-dependent manner. The maximal inhibition was at concentration of 8 μmol / L. Colony formation assay revealed that the combination of ATRA with X-ray treatment significantly reduced the values of D0 and Dq,and shifted down the fitting survival curve,as compared with radiotherapy alone. Moreover,ATAR markedly decreased the percentage of G_2/ M phase in the SGC-7901 cells（ P 0. 05）. In addition,following ATRA treatment,the m RNA levels of Bcl-2 and survivin were decreased（ P 0. 05）,whereas the m RNA levels of Bax and NF-κB were increased（ P 0. 05）.CONCLUSION： ATRA enhances the sensitivity of SGC-7901 cells to radiotherapy,inhibits G_2/ M arrest and regulates the m RNA expression of Bax,Bcl-2,survivin and NF-κB.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第8期1440-1444,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金青年项目(No.81500489)
