摘要
目的:观察全反式维甲酸(All-trans retinoic acid,ATRA)对体外培养人卵巢癌细胞株HO8910增殖、侵袭能力的影响,为ATRA应用于卵巢癌的临床治疗提供实验依据。方法:选用人卵巢癌细胞株HO8910进行体外培养,以不同浓度的ATRA处理HO8910细胞,四甲基偶氮唑蓝(MTT)比色法检测细胞增殖抑制情况,倒置显微镜进行形态学观察,Transwell小室体外侵袭实验检测细胞侵袭能力。结果:(1)ATRA浓度为10-7～10-5mol/L时,均明显抑制HO8910细胞的增殖(P<0.05),并具有时间-剂量依赖性。24、48、72hATRA抑制HO8910细胞增殖的IC50值分别为2.331×10-5、0.998×10-6、0.891×10-7mol/L。(2)倒置显微镜可观察到经10-6mol/LATRA处理的HO8910细胞部分发生形态学的良性分化。(3)体外侵袭实验显示经10-6mol/LATRA处理的HO8910细胞,穿膜细胞数目明显减少(P<0.05)。结论:ATRA能明显抑制人卵巢癌细胞株HO8910的增殖、侵袭能力,有望为ATRA应用于卵巢癌的临床治疗提供新的有效的途径。
Objective：To investigate the effects of all-trans retinoic acid on the human ovarian cancer cell line H08910. Methods： The human ovarian cancer cell line H08910 cultured in vitro were treated with various concentrations of all-trans retinoic acid. The cell proliferation inhibition was detected by MTT assay; The morphologic changes of cell differentiation were observed under inverted microscope; The invasion capability was evaluated by transwell chambers. Results： 1 .ATRA of 10^-7 - 10^-5mol/L inhibited the proliferation of HO8910 cell significantly in a dose-dependent and time-dependent manner （P〈0.05）and its IC50 were 2.331 × 10^-5 mol/L,0.998 × 10^-6 mol/L,0.891 × 10^-7 mol/L at 24 hours,48 hours and 72 hours,separately. 2.Treated by 10^-6 mol/L ATRA, HO8910 cells showed striking morphological characteristics of differentiation under microscope. 3.The number of invasive cells decreased significantly after treated by 10-6 mol/L ATRA （P〈0.05）. Conclusion： ATRA can both inhibit the proliferation and invasion of human ovarian cancer cell line HO8910, Which expect to provide an effective and novel approach for clinical treatment of human ovarian cancer.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第12期1666-1669,共4页
Journal of Chongqing Medical University
关键词
全反式维甲酸
卵巢癌
增殖
侵袭
All-trans retinoic acid
Ovarian carcinoma
Proliferation
Invasion
