摘要
目的:比较淫羊藿的减压提取与常规提取效率。方法:以淫羊藿苷及总黄酮提取率的综合评分为指标,采用L9(34)正交设计法分别优化减压提取与常规提取的工艺,对比提取效率。结果:淫羊藿减压提取的效率明显优于常压提取。最佳常规提取工艺为加10倍量60%乙醇溶液提取3次,每次1 h;最佳减压提取工艺为加10倍量60%乙醇溶液于50℃减压提取3次,每次20 min,真空度0.07 MPa。减压提取1 h与常压提取3 h效果相当,且减压提取干膏得率约降低30%。结论:淫羊藿减压提取工艺优于常规提取工艺,且减压提取工艺稳定,可在生产中推广应用。
Objective：To compare efficiency of vacuum extraction and conventional extraction of Epimedium brevicornum. Method： Taking composite score of icariin and total flavonoids extraction rate as index,L9（34） orthogonal test was used to optimized vacuum extraction and conventional extraction technology of E. brevicornum,and extraction efficiency of them was campared. Result： Extraction efficiency of vacuum extraction was significantly better than that of conventional extraction,optimum vacuum extraction conditions were as followings：extracted 3 times with 10 times the amount of 60% ethanol at 50 ℃,20 minutes each time,vacuum degree 0.07 MPa;Optimum conventional extraction conditions were：extracted 3 times with 10 times the amount of 60% ethanol,1 h each time.Extraction effect of vacuum extraction 1 h and conventional extraction 3 h was equal,and yield of dry extract with vacuum extraction could be reduced by about 30% than that of conventional extraction. Conclusion： Vacuum extraction was superior to conventional extraction technology,and the former was effective and stable,it could be used for industrial production of E. brevicornum.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第5期56-59,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
减压提取
淫羊藿
淫羊藿苷
总黄酮
正交试验
vacuum extraction
Epimedium brevicornum
icariin
total flavonoids
orthogonal test
