摘要
旨在研究日粮中外源添加谷氨酰胺(Glutamine,Glu)对AA肉公鸡生产性能、胴体性状、肌肉品质、肌苷酸含量和H-FABP mRNA表达水平的影响。采用单因子完全随机设计,将240只1d的雄性AA肉仔鸡随机均分为4组(A、B、C和D),分别在基础日粮中添加0、0.2%、0.4%和0.8%水平的谷氨酰胺,21和42d时测定并计算日增体质量、日采食量和料重比;42d时屠宰,测定其剪切力、滴水损失和色度(L*、a*和b*);计算屠宰率、全净膛率、半净膛率、胸肌率、腿肌率和腹脂率;取胸肌和腿肌,用高效液相色谱(HPLC)法测定肌苷酸含量,利用RT-PCR技术检测H-FABP mRNA表达水平的变化。B组可显著提高AA肉鸡的平均日增体质量和平均日采食量(P<0.05),显著增加胸肌、腿肌的肌苷酸含量和H-FABP mRNA的表达水平(P<0.05);C组可显著增加全净膛率、腿肌率、肌间脂肪宽度、胸肌剪切力和减少腹脂率(P<0.05);D组可显著增加腿肌剪切力、胸肌和腿肌的L*和a*值(P<0.05)。日粮中添加0.2%、0.4%和0.8%水平的Glu均可不同程度提高肉鸡生产性能、胴体性状、肌肉品质、肌苷酸的含量和H-FABP mRNA表达水平,在生产中0.2%水平为建议添加量。
The experiment was conducted to determine the effect of exogenous glutamine on the growth performance, carcass traits, meat quality, the content of inosine monophosphate and the expression level of H-FABP in breast and thigh muscle of AA male broilers. A total of 240 AA male broilers were randomly divided into 4 groups （A, B, C and D） which were fed with basic diet supplemented with Glu at 0, 0.2%, 0.4% and 0.8% level, respectively, the average daily gain（ADG）, average daily feed intake（ADFI） and feed/gain ratio（F/G） were determined at 21st and 42nd day, and the experiment lasted for 42 days. The shear force, drip loss and chromal（L＊, a＊ and b＊） were determined and the dressing percentage, eviscerated yield, half eviscerated yield, percentage of breast and thigh muscle were calculated at 42nd day. The content of inosine monophosphate was detected by HPLC, and RT-PCR was used to determine the expression level of H-FABP mRNA in breast and thigh muscle. The average daily gain, average daily intake, the content of inosine monophosphate and the expression level of H-FABP mRNA of broilers were improved significantly in group B（P〈0.05）. The eviscerated yield, percentage of thigh muscle, the breadth of subcutaneous fat, shear force of breast were increased and percentage of abdominal fat were decreased significantly in group C （P〈0.05）. The shear force of thigh, the L＊ and a＊ value of breast and thigh muscle were enhanced significantly in group D （P〈0.05）. The result indicate that Glu supplement at 0.2%, 0.4% and 0.8% levels could improve the growth performance, carcass traits and meat quality of broilers effectively, and the contents of inosine monophosphate and expression level of H-FABP mRNA in breast and thigh muscle could be increased to some extent. The 0.2% level is the recommended dosage in the production.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第11期1740-1746,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
教育部
财政部支持的国家重点专业(教职成厅函2011[71]号)
湖南省青年骨干教师资助(湘教通[2010]345号)
