摘要
为探讨表皮生长因子（EGF）、谷氨酰胺（Gln）和pGRF基因质粒不同组合形式对早期断奶仔猪生产性能、免疫功能及相关激素（皮质醇、生长激素）分泌的影响，选择同日龄断奶仔猪42头，随机分为7个处理，每个处理3个重复，每个重复2头猪，做一对比试验，试验期21d。结果表明，EGF、Gln和pGRF基因质粒的不同组合形式在促进仔猪生长、增强免疫功能及调节激素分泌方面均表现出良好效果，其中以三者联合使用的效果最为突出：仔猪末重和全期日增重分别提高48．02％（P〈0．01）与1．68倍（P〈0．01），料肉比降低50．75％（P〈0．01）；日粮粗蛋白表观消化率增加1．13倍（P〈0．01）；毛色分数提高14．03（P〈0．05），而且全期未发生腹泻，其作用远优于两两组合及各自单独使用。对免疫功能的调节效果以EGF＋pGRF基因质粒和EGF＋G1n＋pGRF基因质粒两种组合形式较为明显：断奶后第1周，试验组血清IgG水平分别比对照组增加11．71％（P〈0．05）和11．24％（P〈0．1）；第2周则分别减少9．53％（P〈0．01）和5．06％（P〈0．1）。对仔猪内分泌的影响，亦以三者联合使用的作用最为显著：其血清皮质醇水平在断奶后1～2周分别比pGRF基因质粒单独使用及其与EGF两者合用降低40．40％和88．57％；生长激素水平的变化与皮质醇呈相反趋势，但变化规律与皮质醇相似。
One growth trial was conducted to investigate the effect of epidermal growth factor （EGF）, glutamine（Gln） and porcine growth release factor gene plasmid （pGRF gene plasmid） on the performance, immune function and the level of growth related hormone of early weaned piglets. In this experiment fortytwo piglets （Landrace× Large White× Taihu, initial weight^# 4.51kg）,weaned at 28 days, were picked out from five litters and allotted randomly to seven equivalent groups according to a one factorial design. The EGF, Gin and pGRF gene plasmid were administrated to all piglets, injected or added to the diets directly, in EGF, Gin, pGRF gene plasmid, EGF＋Gln, EGF＋pGRF gene plasmid and EGF＋Gln＋pGRF gene plasmid treatments respectively. Growth, food consumption, ileal apparent digestibility of protein, index of hair color, diarrhea and contents of immune globulin G （IgG） as well as the growth hormone and glucocorticoid were measured. The simultaneous administration of EGF, Gin and pGRF gene plasmid （EGF＋ Gin＋ pGRF gene plasmid） exerted much better effects than that of the separate use of any of them. This is also true for the combined administration （EGF＋Gln and EGF＋pGRF gene plasmid）. As for the EGF＋ GIn＋pGRF gene plasmid group, the final weight, ADG, ileal apparent digestibility of protein and index of hair color were increased by 48.02% （P〈0.01） and 1.68 times （P〈0.01）, 1.13 times （P〈0.01） and 14.03% （P〈0.05） respectively while the food consumption were decreased by 50.75% （P〈0.01） compared with the control. In addition, there was no diarrhea occurred in this trial. As far as the immune function was concerned, the effects of EGF＋pGRF gene plasmid and EGF＋Gln ＋ pGRF gene plasmid were much better than that of other treatments. As shown in this experiment, the levels of IgG in EGF＋ pGRF gene plasmid and EGF＋Gln＋pGRF gene plasmid groups were increased by 11.71% （P〈0.05） and 11. 24% （P〈0.1） separately in the first week po
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第9期893-899,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家"十五"重点科技攻关项目(2002BA-514A-10-1-4)
