摘要
目的:建立强力健身胶囊定性定量方法。方法:采用薄层色谱法对方中女贞子、远志和甘草进行定性鉴别;采用高效液相色谱法测定样品中淫羊藿苷的含量:色谱柱为Kromasil 100-5 C18(250 mm×4.6 mm,5μm),以乙腈-水(24∶76)为流动相,流速1 mL.min-1,检测波长为270 nm。结果:薄层鉴别的色谱斑点清晰,阴性对照无干扰;淫羊藿苷进样量在0.041～3.318μg的范围内呈良好的线性关系(r=0.9999)。结论:本方法具有简便、准确及专属性强的特点,可用于强力健身胶囊的质量控制。
Objective：To establish the qualitive and quantitative methods for Qiangli Jianshen capsules.Methods：Ligustri Lucidi Fructus,Polygalae Radix,Glycyrrhizae Radix et Rhizoma in the formula were identified qualitatively by TLC and the content of icariin in capsules was determined by HPLC：Kromasil 100-5C18（250 mm×4.6 mm,5 μm） was adopted.The mobile phase consisted of acetonitrile-water（24：76） at a flow rate of 1 mL·min-1.The UV detection wavelength was at 270 nm.Results：TLC spots developed were fairly clear,and the negative control showed no interference.The standard curve of icariin was linear over a range of 0.041-3.318 μg（r=0.9999）.Conclusion：The method is simple,accurate and can be applied to the quality control of Qiangli Jianshen capsules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第4期697-700,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
强力健身胶囊
女贞子
远志
甘草
淫羊藿
淫羊藿苷
薄层色谱法
高效液相色谱法
定性定量
中药分析
Qiangli Jianshen capsules
Ligustri Lucidi Fructus
Polygalae Radix
Glycyrrhizae Radix et Rhizoma
Epimedii Folium
icariin
TLC
HPLC
qualitation and quantitation
analysis of traditional Chinese medicine
