摘要
目的建立参萸清眩膏的薄层色谱法(thin layer chromatography,TLC)鉴别方法。方法采用TLC法对山茱萸、肉苁蓉、枸杞子、熟地黄、菟丝子药材进行鉴别,展开剂分别为甲苯-乙酸乙酯-甲酸(20∶4∶0.5)、氯仿-甲醇(40∶1)、乙酸乙酯-氯仿-甲酸(3∶2∶1)、乙酸乙酯-甲醇-甲酸(16∶0.5∶2)、甲醇-冰醋酸-水(4∶1∶5),薄层板为硅胶G薄层板(10cm×10cm,厚度为0.20~0.25 mm)。结果山茱萸、肉苁蓉、枸杞子、熟地黄、菟丝子的TLC斑点清晰,分离良好,阴性无干扰。结论薄层鉴别方法科学、简便、有效,可用于参萸清眩膏各组分的鉴别。
Objective To establish thin layer chromatography（TLC）method for identification of Shenyuqingxuan Ointment,optimized the quality testing and control.Methods Cornus officinalis,Cistanche deserticola,Fructus lycii,Rehmannia glutinosa and Semen Cuscutae were identified by TLC Shenyuqingxuan Ointment.The spread out agent were methylbenzene-ethyl acetate-formic acid（20∶4∶0.5）,chloroform-methanol（40∶1）,ethyl acetate-chloroform-formic acid（3∶2∶1）,ethyl acetate-methanol-formic acid（16∶0.5∶2）,methanol-glacial acetic acid-water（4∶1∶5）and the thin-layer plate was made with silica gel G（10cm×10cm,0.20-0.25 mm）.Results The TLC spots of Cornus officinalis,Cistanche deserticola,Fructus lycii,Rehmannia glutinosa and Semen Cuscutae were clear,separated well and negative without interference.Conclusion This method is accurate and feasible,which can be applied as the identification of various components in Shenyuqingxuan Ointment.
出处
《华南国防医学杂志》
CAS
2017年第9期631-634,共4页
Military Medical Journal of South China
基金
总后军队医疗机构制剂质量标准计划(14ZJZ06-1)
关键词
薄层色谱法
参萸清眩膏
鉴别
山茱萸
肉苁蓉
Thin layer chromatography
Shenyuqingxuan Ointment
Identification
Cornus officinalis
Cistanchedeserticolo
