摘要
目的:建立高效液相色谱法同时测定补肾益寿片中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的含量。方法:采用EliteKromasil C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈(A)-水(B),梯度洗脱(0～24 min,25%A→27%A;24～30 min,27%A→100%A),流速1 mL.min-1,检测波长270 nm,柱温20℃。结果:朝藿定A进样量在0.096～0.768μg,朝藿定B在0.200～1.60μg,朝藿定C在0.276～2.21μg,淫羊藿苷在0.416～3.33μg范围内呈良好线性关系;相关系数分别为0.9991,0.9986,1.0000,0.9997。朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的平均回收率(n=5)分别为97.8%,99.5%,99.2%,100.2%;RSD分别为2.4%,3.9%,3.1%,3.5%。结论:本文方法可作为补肾益寿片中多指标成分检测的方法,为补肾益寿片的质量控制提供依据。
Objective ： To develop an HPLC method for simultaneous determination of epimedin A, epimedin B, epi- medin C and icariin in Bushenyishou tablets. Methods：The Elite Kromasil C18 （4. 6 mm× 250 ram,5 μm） column was adopted with a mobile phase of acetonitrile （A） -water（ B ） by a gradient eluted program （0 -24 min, 25 % A→ 27%A;24 -30min,27% A→100% A） at the flow rate of 1.0 mL· min^-1. Detection wavelength was set at 270 nm, and the column temperature was set at 20 ℃. Results： The calibration curves showed a good linearity within the ranges of 0. 096 - 0. 768 μg（ r = 0. 9991 ） for epimedin A ,0. 200 - 1.60 μg（ r = 0. 9986） for epimedin B ,0. 276 - 2.21 μg （ r = 1. 0000） for epimedin C, and 0. 416 - 3.33 μg （ r = 0. 9997 ） for icariin. The average recoveries （ n = 5） were 97.8% for epimedin A,99.5% for epimedin B,99.2% for epimedin C ,and 100. 2% for icariin,with RS- Ds of 2.4% ,3.9% ,3.1% and 3.5% , respectively. Conclusion：The method is suitable for the determination of multi - indexed ingredients and quality control of Bushenyishou tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第12期2253-2256,共4页
Chinese Journal of Pharmaceutical Analysis
基金
陕西省科技攻关计划(2008K16-02)
陕西师范大学本科生创新基金
