摘要
目的:建立乳痛宁颗粒的定性定量方法。方法:用薄层色谱法对制剂中的赤芍和首乌藤进行鉴别,其中赤芍的薄层色谱鉴别采用赤芍为对照药材,芍药苷为对照品,以氯仿-乙酸乙酯-甲醇-甲酸(40:5:10:0.2)为展开剂;首乌藤的薄层色谱鉴别采用首乌藤为对照药材,以正己烷-乙酸乙酯-甲酸(30:10:0.5)为展开剂。用高效液相色谱法测定制剂中淫羊藿苷的含量,高效液相的色谱条件为:用 Shimpack CLC-ODS 柱(4.6 mm×150 mm,5μm);流动相为0.7%(v/v)磷酸溶液-乙腈-三乙胺(75:25:0.02);流速为1.0 mL·min^(-1);检测波长为270 nm;柱温为40℃。结果:在薄层色谱中能准确鉴定出制剂中的赤芍和首乌藤;淫羊藿苷进样量在0.078～0.182μg范围内呈良好线性关系(r=0.9993);平均回收率为99.2%(n=9)。结论:本方法可作为乳痛宁颗粒的定性定量方法。
Objective：To establish the quantitative and qualitative methods for Rutongning granules. Methods：TLC was used to identify Radix Paeoniae Rubra and Caulis Polygoni Muhiflori in the Rutongning granules. Chloroform - ethyl acetate - methanol - formic acid （ 40： 5 ： 10.0. 2 ） was used as developing solvent for Radix Paeoniae Rubra with standard crude drug as reference and with paeoniflorin as reference substance. Hexane -ethyl acetate -formic acid（30： 10： 0. 5） was used as developing solvent for Caulis Polygoni Multiflori with standard crude drug as reference. HPLC was used to determine the content of icariine in the prerparation. Conditions of HPLC：using Shimpack CLC - ODS column （4. 6 mm 150 mm, 5 μm） ; mobile phase ： 0. 7 % （v/v） solution of phosphoric acid - acetonitrile - triethylamine （75： 25： 0. 02 ） ; flow rate ： 1.0 mL. min - 1 ; detective wavelength ： 270 nm ; column temperature ： 40 ℃. Results：Radix Paeoniae Rubra and Caulis Polygoni Multiflori were distinguished by TLC. A standard curve for icariine was constructed in the range of 0. 078 - 0. 182 μg （ r = 0. 9993 ）. The average recovery of icariine was 99. 2% （n = 9）. Conclusion：These methods can be used as the quantitative and qualitative methods for Rutongning granules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第2期275-277,共3页
Chinese Journal of Pharmaceutical Analysis
