摘要
目的:建立板桥党参多糖含量测定的方法。方法:采用传统的水提醇沉法提取多糖,石油醚脱脂,savage法脱蛋白;以板桥党参精制多糖测得板桥党参多糖对葡萄糖的换算因子,采用苯酚-硫酸法测定多糖含量。结果:平均加样回收率为98.9%,RSD=1.1%;板桥党参中多糖的含量为19.53%。结论:该法简便、快速、准确、重现性好,为板桥党参的质量控制提供一定依据。
Objective： Establish banqiao radix codonopsis polysaccharide determination method.Methods： water extraction and alcohol sink method to extract polysaccharide,The petroleum ether decolor the colour,ethanol remove the small molecules,savage method to take off protein;The conversion coefficient of banqiao radix codonopsis polysaccharides to glucose was obtained by refined polysaccharides,and then the content of crude polysaccharides in banqiao radix codonopsis was determined by sulfur-icphenol method.Results： The contents of polysaccharide in banqiao radix codonopsis is 19.53%,the average recovery is 98.9%,RSD=1.1%. Conclusion： The method is simple,rapid,accurate,reproducible,it also bring certain basis of the quality control for banqiao radix codonopsis.
出处
《数理医药学杂志》
2011年第6期709-711,共3页
Journal of Mathematical Medicine
关键词
板桥党参
水提醇沉法
苯酚-硫酸法
含量测定
banqiao radix codonopsis
water extraction and alcohol sink method
phenol hydrate-sulfuric
quantative determination
