摘要
目的:研究白芨多糖对HepG2增殖的影响。方法:在HepG2培养基中分别加入终浓度为0、0.5mg/mL、1.0mg/mL、1.5mg/mL的白芨多糖,观察细胞贴壁和形态的变化,采用MTT检测细胞的增殖情况,分别于0、24h、48h对1.0mg/mL白芨多糖处理的细胞进行基因组电泳和caspase-3RT-PCR检测,研究白芨多糖是否可以诱导HepG2凋亡。结果:1.0mg/mL以上浓度的白芨多糖对HepG2贴壁有抑制作用,0~1.5mg/mL白芨多糖不抑制HepG2的分裂,1.0mg/mL的白芨多糖可诱导HepG2细胞凋亡。结论:1.0mg/mL的白芨多糖具有抑制HepG2细胞增殖的作用,作用机理可能为抑制细胞贴壁和诱导HepG2细胞凋亡。
Objective：To investigate the effect of Bletilla striata olysaccharide （BSPS）on the proliferation of HepG2. Methods： BSPS were added into HepG2 cell culture mediumat at the concentration of 0,0.5mg/mL, 1.0mg/mL and 1.5mg/mL respectively, cell attaehment and morphology were observed by microscope and MTT was adopted to detect cell proliferation. DNA break and the expression of caspase-3 were observed by genome electrophoresis and RT-PCR at 0,24h, 48h after treated by BSPS at the concertra- tion of 1.0mg/mL. Results：BSPS inibit cell attaehment at the concertration of more than 1.0mg/mL and didn＇t inhibit cell prolifer- ation at the concentration of 0 and 1.5mg/mL. Cell apoptosis was induced by BSPS with the concentration of 1.0mg/mL. Conclu- sion ： The proliferation of HepG2 was supressed by BSPS through inhibition of cell attaehment and apoptosis induced by BSPS.
出处
《亚太传统医药》
2018年第3期11-13,共3页
Asia-Pacific Traditional Medicine
基金
湖北省大学生创新训练项目(201412310012)
关键词
白芨多糖
HEPG2
增殖
凋亡
Bletilla Striata Polysaccharide
HepG2
Cell Proliferation
Apoptosis
