摘要
目的探讨白芨多糖(Bletilla striata polysaccharide,BSPS)对小鼠免疫功能的调节作用。方法提取白芨多糖并测定其含量。通过腹腔注射环磷酰胺100 mg/(kg.d),连续3 d,建立免疫功能低下小鼠模型,观察灌服不同剂量白芨多糖溶液对免疫功能低下小鼠碳廓清试验的影响;采用MTT法测定白芨多糖对刀豆蛋白A(Concanavalin A,Con A)和脂多糖(Lipopoly-saccharide,LPS)诱导的小鼠脾淋巴细胞增殖的影响。结果提取的3批白芨多糖中的多糖含量分别为93.490%、92.280%和92.629%。在碳廓清试验中,与模型组相比,500 mg/(kg.d)白芨多糖能显著提高免疫抑制小鼠的吞噬指数,其作用与100 mg/(kg.d)的左旋咪唑大致相当。淋巴细胞增殖试验中,1、3、10μg/ml的白芨多糖均可显著提升Con A诱导的小鼠T淋巴细胞增殖的能力,白芨多糖终浓度为3μg/ml时,提升作用最为显著;3μg/ml白芨多糖可显著提升LPS诱导的小鼠B淋巴细胞增殖的能力。结论白芨多糖对小鼠的非特异性免疫和特异性免疫反应均有促进作用。
Objective To investigate the regulatory effect of Bletilla striata polysaccharide（BSPS） on immune function of mice.Methods BSPS was extracted from Bletilla striata and determined for content.Immunosuppressive mouse model was established by intraperitopeal injection with cyclophosphamide at a dosage of 100 mg /（kg·d） for 3 d.The mice were administered intragastrically with BSPS at various dosages and observed for effect on carbon clearance.The effect of BSPS on proliferation levels of splenocytes of mice induced with either concanavalin A（Con A） or lipopolysaccharide（LPS） was determined by MTT method.Results The polysaccharide contents in three batches of extracted BSPS were 93.490%,92.280% and 92.629% respectively.In carbon clearance test,the phagocytic exponent of mice treated with BSPS at a dosage of 500 mg /（kg·d） increased significantly as compared with those in control group,which was equivalent to that treated with levamisole at a dosage of 100 mg /（kg·d）.In lymphocyte proliferation test,the BSPS at dosages of 1,3 and 10μg/ml promoted the proliferation of mouse splenocytes induced by Con A.However,the proliferation level of splenocytes treated with 3μg/ml BSPS was higher than those with BSPS at the other two concentrations.The BSPS at a dosage of 3μg/ml also promoted the proliferation of mouse splenocytes induced by LPS.Conclusion BSPS enhanced both non-specific and specific immune functions of mice.
出处
《中国生物制品学杂志》
CAS
CSCD
2011年第6期676-678,共3页
Chinese Journal of Biologicals
关键词
白芨多糖
小鼠
免疫功能
Bletilla striata polysaccharide（BSPS）
Mouse
Immune function
