摘要
采用荧光光谱和圆二色谱学分析,研究表没食子儿茶素没食子酸酯(EGCG)对嗜水气单胞菌的作用机制。结果表明,EGCG对嗜水气单胞菌的最小抑制浓度为50μg/ml。光谱学分析结果表明EGCG以嵌插方式结合到细菌DNA的双螺旋碱基对中。
EGCG（epigallocatechin-3-gallate） is the main ingredient of green tea extracts and possesses versatile biological activities including broad spectrum antimicrobial activity.In this experiment,antimicrobial activity of EGCG against Aeromonas hydrophila was investigated.As a result,the minimal inhibitory concentration（MIC） of EGCG was 50 μg/ml.The interaction between EGCG and DNA of Aeromona hydrophila in physiological condition（pH 7.4） was studied by fluorescence and CD spectrophotometer.The result of fluorescence spectroscopy demonstrated that EGCG could bind the phosphate group of DNA and be intercalated into the base pairs in a helix of DNA,which would inhibit cellular functions,and ultimately lead to cell death.
出处
《江苏农业学报》
CSCD
北大核心
2011年第5期1128-1132,共5页
Jiangsu Journal of Agricultural Sciences
基金
农业部淡水鱼类遗传育种和养殖生物学重点实验室开放研究基金项目(BZ2009-24)
江苏省自然科学基金项目(BK2010163)
关键词
EGCG
嗜水气单胞菌
基因组DNA
荧光光谱
抑菌机理
epigallocatechin-3-gallate（EGCG）
Aeromonas hydrophila
genomic DNA
fluorescence spectrum
antimicrobial mechanism
