摘要
采用电沉积法将吡啶-4-甲醛缩甲硫氨酸席夫碱铜(Ⅱ)配合物制备[CuL(H2O)2]/GC修饰电极。研究了该电极的电化学性质,发现此电极对还原型谷胱甘肽有良好的电催化氧化作用。考察了修饰电极作为还原型谷胱甘肽传感器的操作条件。结果表明,修饰电极在pH=4.0的磷酸盐缓冲溶液中,于-2～+2V的电位范围内,以100mV/s的扫描速率进行循环伏安扫描,氧化电流峰值与还原型谷胱甘肽浓度在0.2×10-4～0.8×10-4 mol/L范围内呈线性关系,检出限达7.1×10-6 mol/L。该方法已应用于黄瓜中还原型谷胱甘肽含量的测定,其平均含量为0.252×10-4 mol/L,相对标准偏差为4.0%,回收率为95.6%～98.4%。
Shiff-base copper complex was modified on glassy carbon electrode（GCE） through an electro-deposition method.The electro-chemical properties of the electrode was studied and used for the determination of GSH with cyclic voltammetry method.The results showed that in phosphate buffer（pH=4.0）,with linear scan in the range of-0.2~＋0.2 V and a scan rate of 100 mV/s,the catalytic peak had a linear relationship with GSH concentration in the range of 0.2~0.8×10-4 mol／5L-1.The method was used to detect GSH in cucumber,and an average of 0.252×10-4 mol／5L-1 GSH in real sample was obtained,with a relative standard deviation of 4.0% and a detection limit of 7.1×10-6 mol／5L-1.
出处
《分析科学学报》
CAS
CSCD
北大核心
2011年第5期647-650,共4页
Journal of Analytical Science
基金
桂林理工大学博士基金
