摘要
以硒化铜纳米颗粒为能量受体,以罗丹明B为能量供体,二者之间通过光诱导电子转移而猝灭罗丹明B的荧光;而L-半胱氨酸能够诱导罗丹明B荧光的恢复,从而建立了测定L-半胱氨酸的新方法。在p H 4.6、温度为30℃条件下混合2 min后,罗丹明B在575 nm处的荧光强度与溶液中L-半胱氨酸的浓度在2.5×10^(-7)~1.1×10^(-6)mol/L浓度范围内呈良好的线性关系,检出限(3σ/k)为5.5×10^(-8)mol/L。常见的氨基酸对半胱氨酸的测定干扰小,方法快速、选择性好。
The fluorescence of Rhodamine B could be quenched by the manner of photo-induced electron transfer with Cu_（2-x）Se nanoparticles as the energy receptor and Rhodamine B as the energy donor. However,L-cysteine was capable of recovering the fluorescence of Rhodamine B,and the fluorescence intensity was proportional to the concentrations of L-cysteine. Based on that,a novel method for detecting L-cysteine was established. After mixing L-cysteine and Rhodamine B pretreated by Cu_（2-x）Se nanoparticles at p H 4.6 and 30℃for 2 min,a linear relationship was obtained between the fluorescence intensity of Rhodamine B at 575 nm and the concentrations of L-cysteine in the range of 2. 5 × 10^-7- 1. 1 × 10^-6mol / L. This method was used in the determination of L-cysteine with a detection limit（ 3σ / k） of 5. 5 × 10^-8mol / L. The common amino acids presented little interference for the L-cysteine detection.
作者
王雪
杨焜诚
毛智远
黄承志
王健
WANG Xue YANG Kun-Cheng MAO Zhi-Yuan HUANG Cheng-Zhi WANG Jian(Key Laboratory on Luminescence and Real-Time Analytical Chemistry （Southwest University）, Ministry of Education College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2016年第10期1482-1486,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.21405123)
重庆市基础与前沿研究计划项目(No.cstc2014jcyj A50006)
西南大学国家级大学生创新训练项目(No.201610635050)
西南大学药学院大学生创新创业项目资助~~
