摘要
目的:合成仿生量子点脂质体纳米探针(PEG-LP-QDs),优化合成条件并初步探究PEG-LP-QDs的性质,为PEGLP-QDs在体内示踪、肿瘤成像、多重检测等生物医学领域的应用奠定基础。方法:采用薄膜分散法,以磷脂酰胆碱、聚乙二醇(PEG)-磷脂酰乙醇胺、胆固醇为膜材,包裹一定量的油溶性Zn Cd Se/Zn S量子点(QDs)合成PEG-LP-QDs;通过透射电子显微镜(TEM)、纳米颗粒追踪技术(NTA)、荧光分光光度计鉴定并分析PEG-LP-QDs的形貌、粒径和荧光强度,进而优化合成温度及QDs数量。结果:根据合成的LPs粒径与QDs的荧光强度变化,选取40℃为最佳合成温度;TEM和NTA结果证实PEG-LP-QDs合成成功,粒径集中分布于128 nm左右;且当加入10μL浓度为3 mg/m L的QDs时,PEG-LP-QDs的荧光强度达到最大值。结论:成功合成新型PEG-LP-QDs,具有良好的生物相容性和独特的发光特性,其在生物医学领域中的应用价值有待进一步研究。
Objective To synthesize a biomimetic autofluorescence nanoprobe, polyethylene glycol-liposome-quantum dots（PEGLP-QDs）, optimize the synthesis conditions and preliminarily characterize their properties for laying the basis for further application of PEG-LP-QDs in biomedical fields, such as in vivo tracking, tumor detection and multiplex detection. Methods Oil-soluble fluorescent Zn Cd Se/Zn S quantum dots were encapsulated by phosphatidylcholine, PEG-phosphatidyl ethanolamine and cholesterol through thin membrane dispersion method for synthesizing PEG-LP-QDs. Transmission electron microscope,nanoparticle tracking technology and fluorescence spectrophotometer detection methods were used to characterize the morphology,diameter size and fluorescence intensity of PEG-LP-QDs, thus further optimizing the synthesis temperature and QDs quantity.Results As the change of synthesis temperature, the results of LP size-changing and QDs fluorescence-decreasing implied that40 ℃ was a desired temperature. The results of transmission electron microscope and nanoparticle tracking technology proved that PEG-LP-QDs were successfully synthesized. The average diameter size of PEG-LP-QDs was 128 nm. The fluorescence intensity of PEG-LP-QDs reached the maximum when 10 μL 3 mg/m L QDs were encapsulated. Conclusion A novel biomimetic autofluorescence nanoprobe, PEG-LP-QDs, is successfully synthesized, with good biocompatibility and unique luminescent properties. Further work is needed to investigate the application of biomimetic PEG-LP-QDs in biomedical research.
作者
崔曼曼
潘炜伦
刘春辰
李博
CUI Manman;PAN Weilun;LIU Chunchen;LI Bo(Department of Laboratory,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)
出处
《中国医学物理学杂志》
CSCD
2018年第7期849-854,共6页
Chinese Journal of Medical Physics
基金
国家自然科学基金青年项目(81702100)
关键词
纳米探针
脂质体
量子点
仿生膜
nanoprobe
liposomes
quantum dots
biomimetic membrane
