摘要
目的:通过观察中药黄芩的有效成份黄芩苷对人牙周膜细胞(human periodontal ligament cells,hPDLCs)增殖、矿化成骨等功能活性的影响,研究黄芩对人牙周膜细胞的调节作用。方法:采用组织贴块联合胶原酶消化的方法,体外分离、培养、纯化和鉴定hPDLCs后,通过四甲基偶氮唑蓝(MTT)比色法、酶联免疫吸附、半定量逆转录聚合酶链反应(RT-PCR)等方法,检测不同浓度的黄芩苷(10、1.0、0.1、0.01mg/L)对hPDLCs增殖、矿化成骨、骨保护素(osteoprotegerin,OPG)、细胞核因子-κB受体活化因子配基(receptor activator of nuclear factor-κB ligand,RANKL)mRNA表达等方面的影响。结果:0.1mg/L的黄芩苷对于增加人牙周膜细胞的增殖活性、碱性磷酸酶活性和Ⅰ型胶原蛋白的表达作用最强;适宜浓度的黄芩苷可以降低RANKL/OPGmRNA比值。结论:黄芩苷能促进hPDLCs的增殖和成骨作用,该作用可能与RANKL/OPG信号通路相关。
Objective：To observe the effect of baicalin on human periodontal ligament cells＇（hPDLCs） function and activity of proliferation,bone formation and so on.Methods：The hPDLCs＇ abruption,culture,purification and identification was by tissue culture method in the combination of enzyme digestion.To detect the effect of baicalin in different concentrations（10,1.0,0.1,0.01 mg/L） on hPDLCs＇ proliferation,bone formation,osteoprotegerin（OPG）,receptor activator of nuclear factor-κB ligand（RANKL） mRNA expression,we used the methods including MTT method,enzyme linked immunosorbent assay and RT-PCR.Results：The baicalin concentrations of 0.1 mg/L could enhance the proliferation of hPDLCs,activity of alkaline phosphatase（ALP） and expression of collagen protein-Ⅰ,depress the ratio of RANKL/OPG mRNA.Conclusions：Baicalin had the capability of promoting hPDLCs＇ proliferation and bone formation,which reason may be the reduction of RANKL/OPG mRNA.
出处
《口腔生物医学》
2011年第2期62-66,共5页
Oral Biomedicine
基金
国家自然科学基金(30940092)
陕西省"13115"科技创新重大科技专项项目(2009ZDKG-77)
