摘要
目的观察淫羊藿甙对体外培养大鼠成骨细胞护骨素(OPG)、核因子-kB受体活化因子配体(RANKL)mRNA表达的影响。方法用酶消化法分离24h内新生SD大鼠颅盖骨成骨细胞,进行原代培养。在培养液中加入不同浓度的淫羊藿甙(10^(10)～10^(14)mol/L),培养48h后,抽提总RNA,采用半定量RT-PCR法检测成骨细胞中OPG和RANKL mRNA表达的变化。结果淫羊藿甙可明显促进成骨细胞OPG mRNA的表达,抑制RANKL mRNA的表达,且呈浓度依赖性,均以10^(-7)mol/L时作用最显著(P＜0.05)。预先用淫羊藿甙干预成骨细胞,可逆转地塞米松的降低OPG mRNA(0.570±0.093vs 1.083±0.081)、升高RANKL mRNA(1.079±0.050vs 0.718±0.082)的作用(均P＜0.05)。结论淫羊藿甙可以上调OPG基因表达,下调RANKL基因表达,从而调节骨吸收,这可能是淫羊藿甙防治骨质疏松症的重要机制之一。
Objective To investigate the effects of icariin on the expressions of osteoprotegerin （OPG） and receptor activator of nuclear factor-κB ligand （RANKL） mRNA in rat osteoblasts cultured in vitro. Methods Calvarial osteoblasts were obtained from newborn SD rats aged 〈 24 h by trypsin-collagenase digestion method. After treatment with different concentrations of icariin （10^-10-10^-4 mol/L） for 48 h, total RNA was isolated from osteoblasts. Expressions of OPG and RANKL mRNA were detected by semiquantitative RT-PCR. Results Icariin remarkably increased OPG mRNA levels and decreased RANKL mRNA levels in calvarial osteoblasts in a dosedependent fasion with a maximum effect at a concentration of 10^-7 mol/L （P 〈0.05 ）. Osteoblasts pre-treated with icariin prevented the inhibitory effect of dexamethasone on OPG mRNA （0. 570 ± 0. 093 vs 1. 083 ± 0.081 ） and the stimulative effect on RANKL mRNA（1.079 ±0.050 vs 0.718 ±0.082） （both P〈0.05）. Conclusion Icariin upregulates OPG expression and downregulates RANKL expression in osteoblasts, which may explain the beneficial role of icariin in preventing and treating osteoporosis.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2006年第3期222-225,共4页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金(编号30070357)
