摘要
目的观察黄芩苷对高脂血症实验性大鼠肾小球核因子κB及外周血单核细胞趋化蛋白1表达的干预作用。方法实验组动物采用高脂混合饲料+维生素D3喂养方式,建立高脂血症大鼠模型,黄芩苷组在制备模型同时选用20 mg/(kg.d)的黄芩苷水溶液灌胃,对照组喂以标准饲料。4周末测定血脂,取肾小球组织比较病理形态学变化,采用双抗体夹心ELISA法定量分析血清单核细胞趋化蛋白1水平,SABC免疫组织化学法检测肾小球核因子κB表达。结果黄芩苷组大鼠血清中总胆固醇、低密度脂蛋白量减少,单核细胞趋化蛋白1分泌降低,与模型组相比黄芩苷组肾小球病理改变有所减轻,核因子κB表达下调明显,两者间差异有统计学意义(P<0.01)。结论黄芩苷具有降低低密度脂蛋白含量,降低核因子κB在肾小球细胞内激活,下调单核细胞趋化蛋白1表达水平,调节血脂紊乱及抗炎的作用。
Aim To access the effect and mechanisms of baicalin on expression of nuclear factor-κB（NF-κB）and monocyte chemoattractant protein-1（MCP-1） in experimental atherosclerotic rat. Methods High fat forage and vitamin D3 was given by intragastric administration,the experimental group was given baicalein solution 20 mg/（kg·d） for four weeks.The blood lipid,plasma MCP-1 and immunohistochemistry detection of NF-κB on the renal glomerulus was performed. Results The total cholesterol（TC） and low density lipoprotein cholesterol（LDLC） in the serum of rats decreased in the experimental group to 30% baseline values treated by baicalein.The hyperlipidemia markedly raised the activities and contents of MCP-1 in plasma after modeling.Renal interstitium was hyperemic,the glomerular volume became extension,and NF-κB was markedly expressed in the podocyte and tubular epithelial cells than that of the control group（P〈0.01）. Conclusion Baicalein has the function of reducing the levels of LDL,reducing the NF-κB activating in glomerular cells,reducing the performance of MCP-1,and adjusting blood fat disorders and antiinflammatory.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2010年第8期611-613,共3页
Chinese Journal of Arteriosclerosis
基金
国家中医药管理局科学技术研究课题(04-05ZP54)
四川省科技厅项目(03JY029-073-1)
关键词
肾小球
黄芩苷
核因子ΚB
单核细胞趋化蛋白1
Baicalin
Glomcrulus
Nuclear Factor-κB
Monocyte Chemoattractant Protein-1
