摘要
目的: 研究骨碎补总黄酮不同剂量组对成骨细胞体外培养的作用机理。方法: 购买UMA 106 01成骨细胞株进行成骨细胞培养, 观察碱性磷酸酶 (ALP) 活性和3H TdR掺入的情况。结果: 骨碎补总黄酮能提高用UMR 106细胞培养的细胞内ALP活性, 并且在不同时间 24、48、72h有相应变化, 有一定的量效、时效关系, 以 48h最为理想。骨碎补总黄酮对3H TdR的掺入 48h比 24h有明显增加, 有时效关系。结论: 骨碎补总黄酮对成骨细胞分化和增殖均有促进作用。
Objective: To study the mechanism of effect of total flavone of rhizoma drynariae in groups with different dosage on osteoblasts cultured in vitro. Methods: To culture osteoblasts with UMA-106-01 osteoblast strain and to observe the activity of ALP and the endosmosis of 3H-TdR. Results: The total flavone of rhizoma drynariae increased the activity of ALP in cells cultured with UMR-106-01 strain. The ALP activities correspondingly changed in the 24h, 48h and 72h, which related to dosage effect and time effect. Among them, the activity in the 48h is the most ideal one. And the amount of endosmosis of the total flavone of rhizoma drynariae to 3H-TdR increased more remarkably in the 48h than that in the 24h and it also related to the time effect. Conclusion: The total flavone of rhizoma drynariae can promote the differentiation and multiplication of osteoblasts.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2005年第3期161-162,共2页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家"九.五"科技攻关重大疾病课题 (96 906 09 04)
