摘要
目的:探讨miR-146a对小鼠单核-巨噬细胞株RAW264.7以及小鼠原代腹腔巨噬细胞Thl/Th2类细胞因子表达的影响。方法:体外培养的小鼠单核-巨噬细胞系RAW264.7细胞和腹腔新鲜分离的巨噬细胞分别瞬时转染miR-146a mimics、阴性对照mimics(NC mimics)、抑制性miR-146a(miR-146a inhibitor)和抑制性miR-146a阴性对照(NC in-hibitor)。转染后,利用real time PCR定量检测IL-18、IL-5和IL-10的表达情况。结果:RAW264.7细胞和原代腹腔巨噬细胞在转染miR-146a mimics后IL-18的表达水平明显降低(P<0.05),转染miR-146a inhibitor后IL-18的表达水平明显升高(P<0.05),但对IL-5和IL-10的表达,两种转染形式均没有影响。结论:巨噬细胞RAW264.7及原代巨噬细胞表达的miR-146a能够负向调控Thl类细胞因子IL-18的表达,但是不能调控Th2类细胞因子IL-5及IL-10的表达。
AIM： To investigate the effect of miR-146a on the Th1/Th2 cytokine expression in mouse RAW264.7 cell line and primary peritoneal macrophage.METHODS： miR-146a mimics,mimics negative control（NC mimics）,inhibitor miR-146a and inhibitor negative control（NC inhibitor） were transfected into RAW264.7 cells and freshly isolated peritoneal macrophage.IL-18,IL-5 and IL-10 expressions in the cells were measured by real time PCR.RESULTS： MiR-146a mimics suppressed IL-18 expression（P0.05）,and miR-146a specific inhibitor increased IL-18 expression significantly（P0.05）.However,IL-5 and IL-10 expressions were not affected by both miR-146a mimics and miR-146a inhibitor transfections.CONCLUSION： These data demonstrate at first time that miR-146a can regulate Th1 cytokine IL-18 expression,but not affect Th2 cytokine IL-5 and IL-10 expressions.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第5期477-479,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(81001315)
