摘要
目的:建立及改良符合我国萎缩性胃炎(atrophic gastritis,AG)发病特点的大鼠模型并对模型进行评价.方法:健康、清洁级、♂、Wistar大鼠51只,随机分为空白对照组(10只),假手术组(10只),造模2mo组(10只),造模3mo组(10只),造模4mo组(11只),空白组大鼠常规饲养,待造模组大鼠手术完成后,生理盐水灌胃2次/wk,共4wk.假手术组手术过程同造模组但不植入弹簧,术后生理盐水灌胃2次/wk,共4wk.造模2、3、4mo组行弹簧幽门植入术后,予高盐热淀粉糊灌胃2次/wk,分别在灌胃2、3、4mo末取材,放射免疫法测定灌胃4mo末时血清PGE2、胃泌素(gastrin)水平,HE染色法连续观察灌胃2、3、4mo时胃黏膜的病理变化.结果:造模4mo时,与空白对照组和假手术组比较,其血清PGE2、gastrin水平均明显下降(P<0.05或0.01),造模组胃黏膜符合AG表现,且病变随造模时间延长逐渐加重;与空白对照组比较,假手术组、造模2、3、4mo组的胃黏膜体积构成比依次降低3.30%(P>0.05)、9.82%(P<0.05)、17.28%(P<0.01)、20.44%(P<0.01);胃黏膜体积构成比效能单位由低到高为空白对照组(0.000),假手术组(0.1884),模型2mo组(0.527),模型3mo组(0.868),模型4mo组(1.000),胃黏膜萎缩效能(Y,体积变化相对百分百)与对数造模时间(X,h)的时效曲线方程为Y=0.1882+0.9108/[1+10(11.828001-3.709X)](R=0.9992),胃黏膜萎缩半效时间为1545h(95%CI:618.4h-3858h),约2mo.结论:采用弹簧幽门植入术配合高盐热淀粉糊灌胃法制备的大鼠AG模型,符合长期胆汁反流、饮食过热过咸造成的AG,是一种符合我国人群AG发病特点、稳定而便捷的大鼠AG模型.
AIM：To establish a novel rat model of atrophic gastritis according to the characteristics of the disease in Chinese patients.METHODS：Fifty-one healthy male Wistar rats were randomized into five groups：control group（n=10）,sham operation group（n=10）,2-mo model group（n=10）,3-mo model group （n=10）,and 4-mo model group（n=11）.Atrophic gastritis was induced in rats of the three model groups by implanting a spring into the pylorus and intragastrically administering hot salty starch paste.The control group did not underwent any operation and was treated with saline twice a week,i.g.,for 4 wk,while the sham operation group underwent the same operation as model rats except for spring implantation and was treated with saline for the same duration.Serum levels of PGE2 and gastrin were measured by radioimmunoassay.Pathological assessment of gastric changes was performed after intragastric administration.RESULTS：Serum levels of PGE2 and gastrin in the 4-mo model group were lower than those in the control and sham operation groups（all P 0.05 or 0.01）.All the rats of the model groups had histological manifestations in gastric mucosa mimicking atrophic gastritis.These pathological changes deteriorated in a time-dependent manner.The 4-mo model group had the highest constituent ratio of gastric mucosal volume（relative to the control group）,followed by the 3-and 2-mo model groups and sham operation group （20.44%,17.28%,9.82%,3.30%）.The 4-mo model group also had the highest potency unit of gastric mucosa volume,followed by the 3-and 2-mo model groups,sham operation group and control group（1.000,0.868,0.527,0.1884,0.000）.Time-response curve equation of gastric mucosa atrophy potency（Y,volume change relative percentage）and logarithmic time required to induce model（X,h）was Y=0.1882＋0.9108/[1＋10 （11.82800 1-3.709X） ]（R=0.9992）,and half atrophy time was 1 545 h（95%CI：618.4 h-3 858 h）（approximately 2 mo）.CONCLUSION：A rat model of atrophic gastritis was successfully
出处
《世界华人消化杂志》
CAS
北大核心
2011年第10期1001-1008,共8页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30973741~~
