摘要
[目的]观察黄芪、三七及其配伍对慢性萎缩性胃炎大鼠胃黏膜细胞增殖及凋亡的影响。[方法]运用幽门弹簧植入术配合高盐热淀粉糊灌胃法制备萎缩性胃炎大鼠模型,将造模大鼠随机分为模型组、假手术组、黄芪组、三七组、黄芪三七组以及叶酸组,每组10只,另设10只空白组。在治疗阶段,空白组、假手术组和模型组均予生理盐水2mL/d灌胃;黄芪组给予黄芪浸膏3.5g/(kg·d)灌胃;三七组给予三七粉冲剂0.7g/(kg·d)灌胃;黄芪三七组给予黄芪和三七溶液灌胃(其中黄芪3.5g/(kg·d)、三七0.7g/(kg·d),共治疗8周。治疗结束后,应用TUNEL法检测细胞凋亡,免疫组化法检测PCNA蛋白表达,液相芯片技术检测血清fas、fasL、EGF和TGF-β表达。[结果]与空白组比较,模型组大鼠凋亡指数降低(P<0.01),PCNA表达增加(P<0.01),血清fas及fasL均显著升高(P<0.05,P<0.01),血清TGF-β水平降低(P<0.05)。与模型组比较,黄芪三七组、叶酸组可减少凋亡指数,黄芪组和叶酸组可降低PCNA表达,各给药组均能降低血清fas表达(P<0.05,P<0.01),黄芪组及黄芪三七组能显著降低血清fas-L水平(P<0.05)。[结论]黄芪、三七及其配伍可明显改善胃黏膜细胞的增殖及凋亡水平,进一步改善慢性萎缩性胃炎大鼠胃黏膜病变。
[Objective]To evaluate the effect of Astragalus, Panax notoginseng and their compatibility on apoptosis and proliferation of the gastric mucosa in rats with chronic atrophic gastritis（CAG）. [Methods] CAG model was established by implantation of a pyloric spring and intragastic administration of hot starch paste with high-salt. The model rats were randomly divided into model group, sham operation group, folic acid group,Astragalus group,Panax notoginseng group and Astragalus plus Panax notoginseng group. The normal wistar rats were taken as control group. In the 8 weeks therapeutic phase,the control, sham opera tion and model groups were given normal saline 2 mL daily. The Astragalus group was given water decoction of Astragalus containing crude drug 3.5 g/（kg · d）. The Panax notoginseng group was infused with Panax notoginseng powder containing crude drug 0.7 g/（kg· d）. The Astragalus plus Panax notoginseng group was given both Panax notoginseng powder and Astragalus water decoction. TUNEL was used to evaluate gastric mucosal cell apoptosis index and liquid phase chip to evaluate fas,fasl,EGF and TGF-β ex-pression, the protein expression level of PCNA was detected by immunological histological chemistry assay. I-Results]Compared with the control group, the apoptosis index in the model group decreased signifi- cantly（P〈0.01）. The expression of PCNA was significantly increased（P%0.01）, serum levels of fas and fas-L increased significantly（P〈0.01）,serum EGF level was significantly reduced（P〈0.05）. Compared with the model group, the level of fas of all the treatment groups was reduced （P〈0.05, P〈0.01）. The Astragalus group and Astragalus plus Panax notoginseng group reduced the level of fas-L significantly（P〈 0.05）. [Conclusion]Astragalus, Panax notoginseng and their compatibility can effectively protect the gastric mucosal of CAG rats by improving the level of proliferation and apoptosis of gastric mucosal cells.
出处
《中国中西医结合消化杂志》
CAS
2017年第5期376-380,共5页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
国家自然科学基金(No:81373585)
关键词
慢性萎缩性胃炎
黄芪
三七
凋亡
增殖
chronic atrophic gastritis
Astragalus
Panax notoginseng
apoptosis
proliferation
