摘要
目的探讨胃痞康颗粒治疗慢性萎缩性胃炎癌前病变的疗效机理。方法采用甲基硝基亚硝基胍(MNNG)饮水法,造模成功大鼠胃癌前病变模型,用胃痞康颗粒进行逆转性治疗,并与维霉素作对照。各组动物胃黏膜标本,在进行光镜、电镜观测的同时,采用4屏影像MPIAS-500多媒体彩色病理图文分析仪,作DNA含量与倍体检测。结果胃痞康颗粒大、中、小剂量组大鼠胃黏膜细胞DNA平均含量、异倍体发生率(NA%)及核周长、核面积、腺体异形指数均明显小于模型组和维酶素组,2倍体百分率(2C%)则明显高于维酶素组。DNA直方图显示,胃痞素颗粒大、中、小剂量组大鼠细胞核DNA分布窄,集中在2倍体(2C)区域内,峰较单一,维酶素组与模型组大鼠DNA分布明显右移,3-4C、5C增多,甚至出现异倍体(AN),峰相较多,与细胞核DNA含量(U值)增高相吻合。结论胃痞康颗粒治疗慢性萎缩性胃炎的作用机理,与减轻细胞DNA损伤,维持DNA量与质的稳定性有关。
Objective To investigate the mechanism of clinical efficacy of Weipikang granule(WPK)on precancerous lesion of chronic atrophic gastritis.Methods Precancerous lesion of chronic atrophic gastritis model rats were established by adding N-methyl-N -nitro-N nitrosoguanidine(MNNG)in drinking water,and treated with WPK for reverting the lesion.The therapeutic effect was compared to that treated by virginiamycin as control. The gastric mucosa sample was examined under light and electron microscope,and its content...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2005年第S1期113-116,共4页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
胃痞康颗粒
胃肿瘤
癌前病变
DNA含量
形态参数倍体分布
Weipikang granule
gastric cancer
precancerous lesion
DNA content
morphologic parameter
DNA-ploid distribution
