摘要
目的探讨五指毛桃水提液对脾虚模型大鼠胃肠功能的影响。方法将60只大鼠随机分为6组:正常对照组,脾虚模型组,黄芪水提液对照组,五指毛桃水提液高、中、低剂量组,每组10只。除正常对照组外,其余各组大鼠均每天皮下注射利血平1 mg.kg-1复制脾虚模型,脾虚模型组给予纯化水灌胃10 mL.kg-1,黄芪水提液组及五指毛桃水提液高、中、低剂量组同时按上述剂量灌胃给予相应药物。治疗结束后,观察各组大鼠体质量及饮食量变化,酶联免疫吸附测定(ELISA)法检测血浆中β-内啡肽(β-EP)、胃动素(MTL)、胃泌素(GAS)浓度。结果与脾虚模型对照组比较,不同剂量五指毛桃水提液均能明显增加脾虚模型大鼠体质量及饮食量;与正常对照组比较,脾虚模型组大鼠血浆β-EP、MTL、GAS浓度均明显降低(P<0.01);与脾虚模型对照组比较,五指毛桃水提液高、中剂量组大鼠血浆β-EP浓度水平明显上升(P<0.01或P<0.05),五指毛桃水提液高剂量组大鼠血浆MTL浓度水平明显上升(P<0.05),五指毛桃水提液高、低剂量组大鼠血浆GAS浓度水平均明显上升(P<0.05)。结论五指毛桃水提液可能通过调节血浆中β-EP、MTL、GAS浓度水平,有效改善大鼠脾虚证候,这可能是其益气健脾和胃功效治疗脾虚证候的作用机制之一。
Objective To investigate the pharmacologic effect of Radix Fici Hirtae on gastrointestinal function of spleen deficiency syndrome rat model.Methods The rats were randomly divided into blank control group,model control group,Astragalus positive control group,high-does Radix Fici Hirtae aqueous extract,medium-does Radix Fici Hirtae aqueous extract,low-does Radix Fici Hirtae aqueous extract.Excluding the blank control group,the other groups were induced by reserpine to copy of spleen deficiency syndrome models.After the drug treatment,the rats body quality and quantity diet changes were observed in the day of 0 and 14,and Elisa was used to detect the expression of β-EP,MTL,GAS In the plasma.Results Compared with model group,Radix Fici Hirtae aqueous extract could significantly improve the body quality and quantity diet;Compared with blank control group,the levels of β-EP,MTL,GAS were significantly lower in the group of spleen deficiency syndrome model（P〈0.01）;Compared with model control group,the levels of β-EP was significantly higher in high-does and medium-does groups（P〈0.01 or P〈0.05）,the levels of MTL was significantly higher in high-does groups（P〈0.05）,the levels of GAS was significantly higher in high-does and low-does groups（P〈0.05）.Conclusion Radix Fici Hirtae aqueous extract may effectively improve the spleen deficiency of rats by adjusting the levels of β-EP,MTL and GAS,this mayplay a role on treatment spleen deficiency symptoms by the efficacy of spleen-nourishing.
出处
《医药导报》
CAS
北大核心
2012年第10期1264-1267,共4页
Herald of Medicine
关键词
五指毛桃
脾虚模型
Β-内啡肽
胃动素
胃泌素
Radix Fici Hirtae
Spleen deficiency syndrome model
Betaendorphin
Motilin
Gastrin
