摘要
使用重组人结缔组织生长因子（recombinant human connective tissue growth factor,rCTGF）干预体外培养的人成骨细胞,发现rCTGF可呈时间-剂量依赖性地促进人成骨细胞膜型基质金属蛋白酶1及基质金属蛋白酶2的表达,200 ng/ml CTGF作用24～48 h达最大效果.rCTGF可明显增强p38丝裂原活化的蛋白激酶（p38-MAPK）磷酸化,p38-MAPK阻断剂SB 23058可阻断rCTGF上调膜型基质金属蛋白酶1及基质金属蛋白酶2的效应.
Human osteoblast was treated with recombinant human connective tissue growth factor （rCTGF）. This experiment showed that rCTGF increased membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 protein expression in a dose- and time-depentent manner in human osteoblasts. rCTGF induced activation of p38 MAPK in human osteoblasts. p38 MAPK inhibitor SB23058 abrogated the effect of rCTGF on the expressions of membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 in human osteoblasts.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2011年第3期246-249,共4页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金项目（30572078,30600661）
关键词
重组人结缔组织生长因子
成骨细胞
膜型基质金属蛋白酶-1
基质金属蛋白酶-2
Recombinant human connective tissue growth factor
Human osteoblast
Membrane type-1 matrix metalloproteinase
Matrix metalloproteinase-2
