摘要
采用ISSR分子标记技术对黑龙江省25个大豆品种进行遗传多样性分析。结果表明:从28对随机引物中筛选出9对多态性明显、条带清晰、反应稳定的引物;9对引物共扩增出65条带,其中58条为多态性条带,多态性比率为89.23%;Shannon多样性指数平均为0.4604,每个位点的有效等位基因数为1.5139;25个品种间的遗传相似性系数变幅范围为0.4462～0.8923,平均为0.6692;UPGMA聚类分析在GS=0.698处,将25份大豆材料分成5大类。
The genetic diversities of 25 soybean varieties were studied by ISSR markers,9 polymorphic primers were selected from 28 ISSR primers,65 bands were amplified with 9 primers,they contained 58 polymorphic bands and their polymorphism percentage was 89.23%;Shannon＇s index evaluation indicted that average Shannon＇s index was 0.4604 and the numbers of effective alleles were 1.5139;the genetic similarity coefficient among the 25 varieties ranged from 0.4462 to 0.8923,and the average GS was 0.6692;cluster analysis with UPGMA method showed that the cultivars tested in this study could be divided into five groups at the level GS 0.698.
出处
《大豆科学》
CAS
CSCD
北大核心
2011年第1期37-40,共4页
Soybean Science
基金
黑龙江省教育厅科研资助项目(10551331)
